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Literature summary extracted from
- A first step in the development of gene therapy for colorectal carcinoma: Clonig, sequencing, and expression of Escherichia coli cytosine deaminase (1992), Mol. Pharmacol., 43, 380-387.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.4.1 | medicine | gene therapy for treatment of primary and metastatic tumors in the liver, development of gene therapy treatment for metastatic colorectal carcinoma | Escherichia coli |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.4.1 | CD gene codA cloned into mammalian expression vector, WiDr cells, a human CRC cell line transformed | Escherichia coli |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.1 | Fe2+ | catalytically essential divalent metal ion | Escherichia coli |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 52000 | - |
4 * 52000, SDS-PAGE | Escherichia coli |
| 3.5.4.1 | 210000 | - |
- |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.1 | cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.1 | Escherichia coli | - |
Chi 82 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.1 | - |
Escherichia coli |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.1 | 5-fluorocytosine + H2O | - |
Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.4.1 | tetramer | 4 * 52000, SDS-PAGE | Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 60 | - |
thermostable between pH 7-9 | Escherichia coli |
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Release 2023.1 (January 2023)
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