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Literature summary extracted from

  • Yu, T.; Sakai, T.; Omata, S.
    Kinetic properties of cytosine deaminase from Pseudomonas aureofaciens (1976), Agric. Biol. Chem., 40, 551-557.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.1 2-mercaptoethanol 1 mM, relative activity 108% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 alpha,alpha'-dipyridyl 1 mM, relative activity 104% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 ATP
-
 Serratia marcescens
3.5.4.1 ATP 1.0 mM, substrate cytosine, relative activity 105%, substrate 5-methylcytosine relative activity 113% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 dGMP 1.0 mM, substrate cytosine, relative activity 109% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 EDTA 1 mM, relative activity 111% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 ITP
-
 Serratia marcescens
3.5.4.1 ITP
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-amino acids
-
 Serratia marcescens
3.5.4.1 L-amino acids
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-asparagine
-
 Serratia marcescens
3.5.4.1 L-asparagine 10.0 mM, relative activity 156% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-aspartic acid
-
 Serratia marcescens
3.5.4.1 L-aspartic acid 10.0 mM, relative activity 158% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-cysteine 10.0 mM, relative activity 138% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-Glutamic acid 10.0 mM, relative activity 135% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-glutamine 10.0 mM, relative activity 120% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-histidine
-
 Serratia marcescens
3.5.4.1 L-histidine 10.0 mM, relative activity 148% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-hydroxyproline 10.0 mM, relative activity 141% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 L-methionine
-
 Serratia marcescens
3.5.4.1 L-methionine 10.0 mM, relative activity 139% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 NaCN 1 mM, relative activity 104% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 phosphate
-
 Serratia marcescens
3.5.4.1 phosphate 1.0 mM, relative activity 140% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 Sodium azide 1 mM, relative activity 101% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 trichloroacetate 1 mM, relative activity 101% Pseudomonas chlororaphis subsp. aureofaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.1 deoxyguanosine
-
 Serratia marcescens
3.5.4.1 Mn2+
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 p-mercuribenzoate
-
 Pseudomonas chlororaphis subsp. aureofaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.1 0.57
-
 5-Methylcytosine
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 4.5
-
 cytosine
-
 Pseudomonas chlororaphis subsp. aureofaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.1 BaCl2 1.0 mM, relative activity 105% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 CaCl2 1.0 mM, relative activity 113% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 FeCl3
-
 Serratia marcescens
3.5.4.1 FeCl3 1.0 mM, relative activity 130% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 KCl 1.0 mM, relative activity 116% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 NaCl 1.0 mM, relative activity 107% Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 SnCl2 1.0 mM, relative activity 122% Pseudomonas chlororaphis subsp. aureofaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.1 580000
-
-
 Serratia marcescens
3.5.4.1 630000
-
-
 Pseudomonas chlororaphis subsp. aureofaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.1 cytosine + H2O Serratia marcescens
-
 uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O Pseudomonas chlororaphis subsp. aureofaciens
-
 uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O Serratia marcescens type S
-
 uracil + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Pseudomonas chlororaphis subsp. aureofaciens
-
 type P
-
3.5.4.1 Serratia marcescens
-
 type S
-
3.5.4.1 Serratia marcescens type S
-
 type S
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.5.4.1 stable Serratia marcescens
3.5.4.1 unstable Pseudomonas chlororaphis subsp. aureofaciens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.1
-
 Pseudomonas chlororaphis subsp. aureofaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 5-methylcytosine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens thymine + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Serratia marcescens uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Pseudomonas chlororaphis subsp. aureofaciens uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Serratia marcescens type S uracil + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.1 octamer
-
 Serratia marcescens
3.5.4.1 polymer
-
 Pseudomonas chlororaphis subsp. aureofaciens

Synonyms

EC Number Synonyms Comment Organism
3.5.4.1 cytosine deaminase P
-
 Pseudomonas chlororaphis subsp. aureofaciens
3.5.4.1 cytosine deaminase S
-
 Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.1 40 45
-
 Pseudomonas chlororaphis subsp. aureofaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.1 10
-
-
 Pseudomonas chlororaphis subsp. aureofaciens