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Literature summary extracted from

  • Ipata, P.L.; Marmocchi, F.; Magni, G.; Felicioli, R.; Polidoro, G.
    Baker's yeast cytosine deaminase. Some enzymic properties and allosteric inhibition by nucleosides and nucleotides (1971), Biochemistry, 10, 4270-4276.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.1 CDP
-
 Saccharomyces cerevisiae
3.5.4.1 CMP
-
 Saccharomyces cerevisiae
3.5.4.1 CTP
-
 Saccharomyces cerevisiae
3.5.4.1 cytidine
-
 Saccharomyces cerevisiae
3.5.4.1 GDP
-
 Saccharomyces cerevisiae
3.5.4.1 GMP
-
 Saccharomyces cerevisiae
3.5.4.1 GTP
-
 Saccharomyces cerevisiae
3.5.4.1 guanosine
-
 Saccharomyces cerevisiae
3.5.4.1 p-mercuribenzoate 0.3 mM, 50% inhibition Saccharomyces cerevisiae
3.5.4.1 thymidine
-
 Saccharomyces cerevisiae
3.5.4.1 TTP
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.1 2.5
-
 cytosine
-
 Saccharomyces cerevisiae
3.5.4.1 2.5
-
 5-Methylcytosine
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.1 34000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.1 cytosine + H2O Escherichia coli
-
 uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O Saccharomyces cerevisiae
-
 uracil + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Escherichia coli
-
-
-
3.5.4.1 Saccharomyces cerevisiae
-
 baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.1 partially Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.1 3.1
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.5.4.1 4°C, stable in the pH-range 5-9 for at least 48 h Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 5-methylcytosine + H2O
-
 Saccharomyces cerevisiae thymine + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Escherichia coli uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Saccharomyces cerevisiae uracil + NH3
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.1 7
-
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.4.1 5 9 stable in this range at 4°C for at least 48 h Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.4.1 0.3
-
 p-mercuribenzoate
-
 Saccharomyces cerevisiae