BRENDA - Enzyme Database

Guanidinobutyrase for L-arginine degradation in Brevibacterium helvolum

Yorifuji, T.; Shimizu, E.; Hirata, H.; Imada, K.; Katsumi, T.; Sawamura, S.; Biosci. Biotechnol. Biochem. 56, 773-777 (1992)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.7
2-mercaptoethanol
-
Brevibacterium helvolum
3.5.3.7
2-mercaptopropionate
-
Brevibacterium helvolum
3.5.3.7
3-mercaptoethylamine
-
Brevibacterium helvolum
3.5.3.7
3-Mercaptopropionate
-
Brevibacterium helvolum
3.5.3.7
5-Aminovalerate
-
Brevibacterium helvolum
3.5.3.7
6-aminocaproate
-
Brevibacterium helvolum
3.5.3.7
agmatine
-
Brevibacterium helvolum
3.5.3.7
mercaptoethylamine
-
Brevibacterium helvolum
3.5.3.7
n-Butyrate
-
Brevibacterium helvolum
3.5.3.7
n-valerate
-
Brevibacterium helvolum
3.5.3.7
o-phenanthroline
-
Brevibacterium helvolum
3.5.3.7
thioglycolate
-
Brevibacterium helvolum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.7
2.9
-
4-Guanidinobutyrate
-
Brevibacterium helvolum
3.5.3.7
71
-
L-arginine
-
Brevibacterium helvolum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.7
Zn2+
firmly bound
Brevibacterium helvolum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.7
45000
-
4 * 45000, SDS-PAGE
Brevibacterium helvolum
3.5.3.7
65000
-
2 * 65000, room temperature, SDS-PAGE, compactly associated subunits
Brevibacterium helvolum
3.5.3.7
190000
-
gel filtration
Brevibacterium helvolum
3.5.3.7
194000
-
gel filtration
Brevibacterium helvolum
3.5.3.7
210000
-
gel filtration, undenatured enzyme
Brevibacterium helvolum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.7
4-Guanidinobutyrate + H2O
Pseudomonas fluorescens
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Pimelobacter simplex
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Brevibacterium linens
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Arthrobacter globiformis
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Streptomyces griseus
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Brevibacterium helvolum
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Corynebacterium ammoniagenes
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Brevibacterium lipolyticum
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Corynebacterium stationis
-
4-Aminobutyrate + urea
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.7
Arthrobacter globiformis
-
IFO 12137 (ATCC 8010)
-
3.5.3.7
Brevibacterium helvolum
-
IFO 12073 (ATCC 11822)
-
3.5.3.7
Brevibacterium linens
-
IFO 12141
-
3.5.3.7
Brevibacterium lipolyticum
-
IFO 12678
-
3.5.3.7
Corynebacterium ammoniagenes
-
IFO 12072 (ATCC 6872)
-
3.5.3.7
Corynebacterium stationis
-
IFO 12144
-
3.5.3.7
Pimelobacter simplex
-
IFO 12069 (ATCC 6946)
-
3.5.3.7
Pseudomonas fluorescens
-
-
-
3.5.3.7
Streptomyces griseus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.7
-
Brevibacterium helvolum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.3.7
83.8
-
-
Brevibacterium helvolum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Pseudomonas fluorescens
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Pimelobacter simplex
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Brevibacterium linens
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Arthrobacter globiformis
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Streptomyces griseus
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Brevibacterium helvolum
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Corynebacterium ammoniagenes
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Brevibacterium lipolyticum
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Corynebacterium stationis
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
arginine + H2O
weak activity towards L- and D-arginine
209481
Brevibacterium helvolum
2,5-diaminopentanoate + urea
-
-
-
?
3.5.3.7
L-canavanine + H2O
-
209481
Brevibacterium helvolum
L-2-amino-4-(aminooxy)butanoic acid + urea
-
-
-
?
3.5.3.7
additional information
-
209481
Streptomyces griseus
?
-
-
-
-
3.5.3.7
additional information
guanidinoacetate, L-guanidinosuccinate, 6-guanidinocaproate, 2-guanidinoethyl sulfate, 4-guanidinobutyramide, taurocyamine, creatine, methylguanidine, N-amidino-L-alanine and agmatine are inert as substrates
209481
Brevibacterium helvolum
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.7
dimer
2 * 65000, room temperature, SDS-PAGE, compactly associated subunits
Brevibacterium helvolum
3.5.3.7
tetramer
4 * 45000, SDS-PAGE
Brevibacterium helvolum
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.5.3.7
50
-
residual activity after heating for 60 min 50% of the original activity
Brevibacterium helvolum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.7
9
-
-
Brevibacterium helvolum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.7
2-mercaptoethanol
-
Brevibacterium helvolum
3.5.3.7
2-mercaptopropionate
-
Brevibacterium helvolum
3.5.3.7
3-mercaptoethylamine
-
Brevibacterium helvolum
3.5.3.7
3-Mercaptopropionate
-
Brevibacterium helvolum
3.5.3.7
5-Aminovalerate
-
Brevibacterium helvolum
3.5.3.7
6-aminocaproate
-
Brevibacterium helvolum
3.5.3.7
agmatine
-
Brevibacterium helvolum
3.5.3.7
mercaptoethylamine
-
Brevibacterium helvolum
3.5.3.7
n-Butyrate
-
Brevibacterium helvolum
3.5.3.7
n-valerate
-
Brevibacterium helvolum
3.5.3.7
o-phenanthroline
-
Brevibacterium helvolum
3.5.3.7
thioglycolate
-
Brevibacterium helvolum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.7
2.9
-
4-Guanidinobutyrate
-
Brevibacterium helvolum
3.5.3.7
71
-
L-arginine
-
Brevibacterium helvolum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.7
Zn2+
firmly bound
Brevibacterium helvolum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.7
45000
-
4 * 45000, SDS-PAGE
Brevibacterium helvolum
3.5.3.7
65000
-
2 * 65000, room temperature, SDS-PAGE, compactly associated subunits
Brevibacterium helvolum
3.5.3.7
190000
-
gel filtration
Brevibacterium helvolum
3.5.3.7
194000
-
gel filtration
Brevibacterium helvolum
3.5.3.7
210000
-
gel filtration, undenatured enzyme
Brevibacterium helvolum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.7
4-Guanidinobutyrate + H2O
Pseudomonas fluorescens
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Pimelobacter simplex
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Brevibacterium linens
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Arthrobacter globiformis
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Streptomyces griseus
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Brevibacterium helvolum
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Corynebacterium ammoniagenes
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Brevibacterium lipolyticum
-
4-Aminobutyrate + urea
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
Corynebacterium stationis
-
4-Aminobutyrate + urea
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.7
-
Brevibacterium helvolum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.3.7
83.8
-
-
Brevibacterium helvolum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Pseudomonas fluorescens
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Pimelobacter simplex
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Brevibacterium linens
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Arthrobacter globiformis
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Streptomyces griseus
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Brevibacterium helvolum
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Corynebacterium ammoniagenes
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Brevibacterium lipolyticum
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
4-Guanidinobutyrate + H2O
-
209481
Corynebacterium stationis
4-Aminobutyrate + urea
-
-
-
?
3.5.3.7
arginine + H2O
weak activity towards L- and D-arginine
209481
Brevibacterium helvolum
2,5-diaminopentanoate + urea
-
-
-
?
3.5.3.7
L-canavanine + H2O
-
209481
Brevibacterium helvolum
L-2-amino-4-(aminooxy)butanoic acid + urea
-
-
-
?
3.5.3.7
additional information
-
209481
Streptomyces griseus
?
-
-
-
-
3.5.3.7
additional information
guanidinoacetate, L-guanidinosuccinate, 6-guanidinocaproate, 2-guanidinoethyl sulfate, 4-guanidinobutyramide, taurocyamine, creatine, methylguanidine, N-amidino-L-alanine and agmatine are inert as substrates
209481
Brevibacterium helvolum
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.7
dimer
2 * 65000, room temperature, SDS-PAGE, compactly associated subunits
Brevibacterium helvolum
3.5.3.7
tetramer
4 * 45000, SDS-PAGE
Brevibacterium helvolum
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.5.3.7
50
-
residual activity after heating for 60 min 50% of the original activity
Brevibacterium helvolum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.7
9
-
-
Brevibacterium helvolum