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Literature summary extracted from

  • Yorifuji, T.; Shimizu, E.; Hirata, H.; Imada, K.; Katsumi, T.; Sawamura, S.
    Guanidinobutyrase for L-arginine degradation in Brevibacterium helvolum (1992), Biosci. Biotechnol. Biochem., 56, 773-777.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.7 2-mercaptoethanol
-
 Brevibacterium helvolum
3.5.3.7 2-mercaptopropionate
-
 Brevibacterium helvolum
3.5.3.7 3-mercaptoethylamine
-
 Brevibacterium helvolum
3.5.3.7 3-Mercaptopropionate
-
 Brevibacterium helvolum
3.5.3.7 5-Aminovalerate
-
 Brevibacterium helvolum
3.5.3.7 6-aminocaproate
-
 Brevibacterium helvolum
3.5.3.7 agmatine
-
 Brevibacterium helvolum
3.5.3.7 mercaptoethylamine
-
 Brevibacterium helvolum
3.5.3.7 n-Butyrate
-
 Brevibacterium helvolum
3.5.3.7 n-valerate
-
 Brevibacterium helvolum
3.5.3.7 o-phenanthroline
-
 Brevibacterium helvolum
3.5.3.7 thioglycolate
-
 Brevibacterium helvolum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.7 2.9
-
 4-Guanidinobutyrate
-
 Brevibacterium helvolum
3.5.3.7 71
-
 L-arginine
-
 Brevibacterium helvolum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.7 Zn2+ firmly bound Brevibacterium helvolum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.7 45000
-
 4 * 45000, SDS-PAGE Brevibacterium helvolum
3.5.3.7 65000
-
 2 * 65000, room temperature, SDS-PAGE, compactly associated subunits Brevibacterium helvolum
3.5.3.7 190000
-
 gel filtration Brevibacterium helvolum
3.5.3.7 194000
-
 gel filtration Brevibacterium helvolum
3.5.3.7 210000
-
 gel filtration, undenatured enzyme Brevibacterium helvolum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas fluorescens
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pimelobacter simplex
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Brevibacterium linens
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Arthrobacter globiformis
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Streptomyces griseus
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Brevibacterium helvolum
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Corynebacterium ammoniagenes
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Brevibacterium lipolyticum
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Corynebacterium stationis
-
 4-Aminobutyrate + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.7 Arthrobacter globiformis
-
 IFO 12137 (ATCC 8010)
-
3.5.3.7 Brevibacterium helvolum
-
 IFO 12073 (ATCC 11822)
-
3.5.3.7 Brevibacterium linens
-
 IFO 12141
-
3.5.3.7 Brevibacterium lipolyticum
-
 IFO 12678
-
3.5.3.7 Corynebacterium ammoniagenes
-
 IFO 12072 (ATCC 6872)
-
3.5.3.7 Corynebacterium stationis
-
 IFO 12144
-
3.5.3.7 Pimelobacter simplex
-
 IFO 12069 (ATCC 6946)
-
3.5.3.7 Pseudomonas fluorescens
-
-
-
3.5.3.7 Streptomyces griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.7
-
 Brevibacterium helvolum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.7 83.8
-
-
 Brevibacterium helvolum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas fluorescens 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pimelobacter simplex 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Brevibacterium linens 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Arthrobacter globiformis 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Streptomyces griseus 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Brevibacterium helvolum 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Corynebacterium ammoniagenes 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Brevibacterium lipolyticum 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Corynebacterium stationis 4-Aminobutyrate + urea
-
 ?
3.5.3.7 arginine + H2O weak activity towards L- and D-arginine Brevibacterium helvolum 2,5-diaminopentanoate + urea
-
 ?
3.5.3.7 L-canavanine + H2O
-
 Brevibacterium helvolum L-2-amino-4-(aminooxy)butanoic acid + urea
-
 ?
3.5.3.7 additional information
-
 Streptomyces griseus ?
-
 ?
3.5.3.7 additional information guanidinoacetate, L-guanidinosuccinate, 6-guanidinocaproate, 2-guanidinoethyl sulfate, 4-guanidinobutyramide, taurocyamine, creatine, methylguanidine, N-amidino-L-alanine and agmatine are inert as substrates Brevibacterium helvolum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.7 dimer 2 * 65000, room temperature, SDS-PAGE, compactly associated subunits Brevibacterium helvolum
3.5.3.7 tetramer 4 * 45000, SDS-PAGE Brevibacterium helvolum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.7 50
-
 residual activity after heating for 60 min 50% of the original activity Brevibacterium helvolum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.7 9
-
-
 Brevibacterium helvolum