Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Misawa, S.; Aoshima, M.; Takaku, H.; Matsumoto, M.; Hayashi, H.
    High-level expression of Mycoplasma arginine deiminase in Escherichia coli and its efficient renaturation as an anti-tumor enzyme (1994), J. Biotechnol., 36, 145-155.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.6 expression in the cytosol of Escherichia coli Mycoplasmopsis arginini

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.6 L-Arg + H2O Mycoplasmopsis arginini the recombinant enzyme inhibits the growth of two mouse cells lines hepatoma MH134 and fibrosarcoma Meth A citrulline + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.6 Mycoplasmopsis arginini
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.6 recombinant enzyme Mycoplasmopsis arginini

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.3.6 renaturation after treatment with 6 M guanidine hydrochloride: optimum renaturation conditions are incubation for 90 h at pH 7.5 and 15°C Mycoplasmopsis arginini

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.6 33.6
-
-
 Mycoplasmopsis arginini

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.6 L-Arg + H2O
-
 Mycoplasmopsis arginini L-citrulline + NH3
-
 ?
3.5.3.6 L-Arg + H2O the recombinant enzyme inhibits the growth of two mouse cells lines hepatoma MH134 and fibrosarcoma Meth A Mycoplasmopsis arginini citrulline + NH3
-
 ?