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Literature summary extracted from

  • Shibatani, T.; Kakimoto, T.; Chibata, I.
    Crystallization and properties of L-arginine deiminase of Pseudomonas putida (1975), J. Biol. Chem., 250, 4580-4583.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.3.6
-
 Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.6 Ag+
-
 Pseudomonas putida
3.5.3.6 Al3+
-
 Pseudomonas putida
3.5.3.6 Cu2+
-
 Pseudomonas putida
3.5.3.6 Fe2+
-
 Pseudomonas putida
3.5.3.6 Fe3+
-
 Pseudomonas putida
3.5.3.6 Hg2+
-
 Pseudomonas putida
3.5.3.6 L-Alpha-amino-beta-guanidinopropionic acid competitive Pseudomonas putida
3.5.3.6 L-Alpha-amino-gamma-guanidinobutyric acid competitive Pseudomonas putida
3.5.3.6 L-homoarginine competitive Pseudomonas putida
3.5.3.6 PCMB
-
 Pseudomonas putida
3.5.3.6 Sn2+
-
 Pseudomonas putida
3.5.3.6 Zn2+
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.6 0.2
-
 L-Arg
-
 Pseudomonas putida
3.5.3.6 2.38
-
 L-Alpha-amino-beta-guanidinopropionic acid
-
 Pseudomonas putida
3.5.3.6 3.03
-
 L-Alpha-amino-gamma-guanidinobutyric acid
-
 Pseudomonas putida
3.5.3.6 3.38
-
 L-nitroarginine
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.6 54000
-
 2 * 54000, SDS-PAGE Pseudomonas putida
3.5.3.6 120000
-
 equilibrium sedimentation Pseudomonas putida
3.5.3.6 130000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.6 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.6
-
 Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.6 58.8
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
3.5.3.6 4°C, pH 8.0, stable for 1 week Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.6 L-alpha-amino-beta-guanidinepropionic acid + H2O
-
 Pseudomonas putida 2-amino-3-carbamoylpropionic acid + NH3
-
 ?
3.5.3.6 L-alpha-amino-beta-guanidinopropionic acid + H2O
-
 Pseudomonas putida 2-amino-3-carbamoylpropionic acid + NH3
-
 ?
3.5.3.6 L-alpha-amino-gamma-guanidinobutyric acid + H2O
-
 Pseudomonas putida 2-amino-4-carbamoylbutanoic acid + NH3
-
 ?
3.5.3.6 L-Arg + H2O
-
 Pseudomonas putida L-citrulline + NH3
-
 ?
3.5.3.6 L-nitroarginine + H2O
-
 Pseudomonas putida NH3 + nitrocitrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.6 dimer 2 * 54000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.6 50
-
-
 Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.3.6 35 53 35°C: about 55% of maximal activity, 53°C: about 50% of maximal activity Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.6 40
-
 1 h, stable Pseudomonas putida
3.5.3.6 55
-
 3 min, 50% loss of activity Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.6 6
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.6 5 7.5 pH 5.0: about 40% of maximal activity, pH 7.5: about 50% of maximal activity Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.6 6
-
 rapid loss of activity Pseudomonas putida
3.5.3.6 8
-
 4°C, stable for 1 week Pseudomonas putida