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Literature summary extracted from

  • Yorifuji, T.; Komaki, N.; Oketani, K.; Entani, E.
    Guanidinoacetate amidinohydrolase from a Flavobacterium. Purification, crystallization and properties (1979), Agric. Biol. Chem., 43, 55-62.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.3.2
-
 Flavobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.2 1,10-phenanthroline
-
 Flavobacterium sp.
3.5.3.2 DTNB inhibits the reaction slightly Flavobacterium sp.
3.5.3.2 p-chloromercuribenzoate
-
 Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.2 15
-
 Guanidinoacetate
-
 Flavobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.2 Co2+
-
 Flavobacterium sp.
3.5.3.2 Zn2+ restores activity of 1,10-phenanthroline treated enzyme Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.2 70000
-
 4 * 70000, SDS-PAGE Flavobacterium sp.
3.5.3.2 281000
-
 gel filtration, disc gel electrophoreisis Flavobacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.2 guanidinoacetate + H2O Pseudomonas putida
-
 glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O Flavobacterium sp.
-
 glycine + urea
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.2 Flavobacterium sp.
-
 F. sp. GE-1
-
3.5.3.2 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.2
-
 Flavobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.2 190
-
-
 Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.2 3-guanidinopropionate + H2O
-
 Flavobacterium sp. 3-aminopapanoate + urea
-
 ?
3.5.3.2 4-guanidinobutyrate + H2O
-
 Pseudomonas putida 4-aminobutanoate + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O
-
 Pseudomonas putida glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O
-
 Flavobacterium sp. glycine + urea
-
 ir
3.5.3.2 additional information N-amidinoalanine, L-arginine, agmatine and creatine are no substrates Flavobacterium sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.2 tetramer 4 * 70000, SDS-PAGE Flavobacterium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.2 60.2
-
 Guanidinoacetate
-
 Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.2 8 8.5
-
 Flavobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.2 4 11
-
 Flavobacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.2 5.5 10 stable in this range Flavobacterium sp.