BRENDA - Enzyme Database

A new amidinohydrolase, methylguanidine amidinohydrolase from Alcaligenes sp. N-42

Nakajima, M.; Shirokane, Y.; Mizusawa, K.; FEBS Lett. 110, 43-46 (1980)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.5.3.16
medicine
useful as a means of detoxication and/or assay of methylguanidine in body fluids
Alcaligenes sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.16
methylguanidine + H2O
Alcaligenes sp.
-
methylamine + urea
-
-
?
3.5.3.16
methylguanidine + H2O
Alcaligenes sp. N-42
-
methylamine + urea
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.16
Alcaligenes sp.
-
N-42
-
3.5.3.16
Alcaligenes sp. N-42
-
N-42
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.16
-
Alcaligenes sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.16
102
-
-
Alcaligenes sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.16
agmatine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
?
-
-
-
?
3.5.3.16
ethylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
ethylamine + urea
-
-
-
?
3.5.3.16
ethylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
ethylamine + urea
-
-
-
?
3.5.3.16
guanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
NH3 + urea
-
-
-
?
3.5.3.16
methylguanidine + H2O
-
209433
Alcaligenes sp.
methylamine + urea
-
-
-
?
3.5.3.16
methylguanidine + H2O
-
209433
Alcaligenes sp. N-42
methylamine + urea
-
-
-
?
3.5.3.16
additional information
no hydrolysis of guanidino compounds with carboxyl group
209433
Alcaligenes sp.
?
-
-
-
-
3.5.3.16
additional information
no hydrolysis of guanidino compounds with carboxyl group
209433
Alcaligenes sp. N-42
?
-
-
-
-
3.5.3.16
n-butylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
n-butylamine + urea
-
-
-
?
3.5.3.16
n-butylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
n-butylamine + urea
-
-
-
?
3.5.3.16
n-propylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
n-propylamine + urea
-
-
-
?
3.5.3.16
n-propylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
n-propylamine + urea
-
-
-
?
Application (protein specific)
EC Number
Application
Commentary
Organism
3.5.3.16
medicine
useful as a means of detoxication and/or assay of methylguanidine in body fluids
Alcaligenes sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.16
methylguanidine + H2O
Alcaligenes sp.
-
methylamine + urea
-
-
?
3.5.3.16
methylguanidine + H2O
Alcaligenes sp. N-42
-
methylamine + urea
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.16
-
Alcaligenes sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.16
102
-
-
Alcaligenes sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.16
agmatine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
?
-
-
-
?
3.5.3.16
ethylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
ethylamine + urea
-
-
-
?
3.5.3.16
ethylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
ethylamine + urea
-
-
-
?
3.5.3.16
guanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
NH3 + urea
-
-
-
?
3.5.3.16
methylguanidine + H2O
-
209433
Alcaligenes sp.
methylamine + urea
-
-
-
?
3.5.3.16
methylguanidine + H2O
-
209433
Alcaligenes sp. N-42
methylamine + urea
-
-
-
?
3.5.3.16
additional information
no hydrolysis of guanidino compounds with carboxyl group
209433
Alcaligenes sp.
?
-
-
-
-
3.5.3.16
additional information
no hydrolysis of guanidino compounds with carboxyl group
209433
Alcaligenes sp. N-42
?
-
-
-
-
3.5.3.16
n-butylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
n-butylamine + urea
-
-
-
?
3.5.3.16
n-butylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
n-butylamine + urea
-
-
-
?
3.5.3.16
n-propylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
n-propylamine + urea
-
-
-
?
3.5.3.16
n-propylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
n-propylamine + urea
-
-
-
?