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Literature summary extracted from

  • Ohsugi, I.; Takahara, H.; Shiraiwa, M.; Sugawara, K.
    Expression of mouse uterine peptidylarginine deiminase in Escherichia coli: construction of expression plasmid and properties of the recombinant enzyme (1995), Arch. Biochem. Biophys., 317, 62-68.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.15 expression in Escherichia coli Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.15 0.5
-
 benzoyl-Arg ethyl ester native enzyme Mus musculus
3.5.3.15 0.53
-
 benzoyl-L-Arg methyl ester recombinant enzyme Mus musculus
3.5.3.15 0.54
-
 benzoyl-L-Arg methyl ester native enzyme Mus musculus
3.5.3.15 0.54
-
 benzoyl-Arg ethyl ester recombinant enzyme Mus musculus
3.5.3.15 1.37
-
 benzoyl-L-Arg-NH2 native enzyme Mus musculus
3.5.3.15 1.39
-
 benzoyl-L-Arg-NH2 recombinant enzyme Mus musculus
3.5.3.15 2.28
-
 benzoyl-L-Arg native enzyme Mus musculus
3.5.3.15 2.58
-
 benzoyl-L-Arg recombinant enzyme Mus musculus
3.5.3.15 2.96
-
 acetyl-L-Arg methyl ester native enzyme Mus musculus
3.5.3.15 3.24
-
 acetyl-L-Arg methyl ester recombinant enzyme Mus musculus
3.5.3.15 10.67
-
 acetyl-L-Arg recombinant enzyme Mus musculus
3.5.3.15 11.3
-
 acetyl-L-Arg native enzyme Mus musculus
3.5.3.15 23.49
-
 L-Arg methyl ester recombinant enzyme Mus musculus
3.5.3.15 26.45
-
 L-Arg methyl ester native enzyme Mus musculus
3.5.3.15 33.4
-
 L-Arg native enzyme Mus musculus
3.5.3.15 36.3
-
 L-Arg recombinant enzyme Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.15 Ca2+ maximal activity at 2 mM Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.15 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.15 recombinant enzyme Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.15 muscle skeletal muscle Mus musculus
-
3.5.3.15 uterus
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.15 8.45
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.15 acety-L-Arg + H2O
-
 Mus musculus acetyl-L-citrulline + NH3
-
 ?
3.5.3.15 acetyl-L-Arg + H2O
-
 Mus musculus acetyl-L-citrulline + NH3
-
 ?
3.5.3.15 benzoyl-Arg ethyl ester + H2O
-
 Mus musculus benzoyl-citrulline ethyl ester + NH3
-
 ?
3.5.3.15 benzoyl-Arg methyl ester + H2O
-
 Mus musculus benzoyl-citrulline methyl ester + NH3
-
 ?
3.5.3.15 benzoyl-L-Arg + H2O
-
 Mus musculus benzoyl-L-citrulline + NH3
-
 ?
3.5.3.15 L-Arg + H2O
-
 Mus musculus L-citrulline + NH3
-
 ?
3.5.3.15 L-Arg methyl ester + H2O
-
 Mus musculus L-citrulline methyl ester + NH4+
-
 ?
3.5.3.15 N-acetyl-Arg methyl ester + H2O
-
 Mus musculus N-acetyl-citrulline + NH4+
-
 ?
3.5.3.15 Nalpha-benzoyl-L-arginine ethyl ester + H2O
-
 Mus musculus Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.15 0.17
-
 L-Arg methyl ester recombinant enzyme Mus musculus
3.5.3.15 0.22
-
 L-Arg recombinant enzyme Mus musculus
3.5.3.15 0.31
-
 L-Arg methyl ester native enzyme Mus musculus
3.5.3.15 0.32
-
 acetyl-L-Arg recombinant enzyme Mus musculus
3.5.3.15 0.37
-
 acetyl-L-Arg native enzyme Mus musculus
3.5.3.15 0.52
-
 L-Arg native enzyme Mus musculus
3.5.3.15 1.11
-
 benzoyl-L-Arg native enzyme Mus musculus
3.5.3.15 1.41
-
 benzoyl-L-Arg recombinant enzyme Mus musculus
3.5.3.15 4.11
-
 acetyl-L-Arg methyl ester native enzyme Mus musculus
3.5.3.15 4.41
-
 acetyl-L-Arg methyl ester recombinant enzyme Mus musculus
3.5.3.15 5.31
-
 benzoyl-L-Arg-NH2 recombinant enzyme Mus musculus
3.5.3.15 5.51
-
 benzoyl-L-Arg-NH2 native enzyme Mus musculus
3.5.3.15 5.69
-
 benzoyl-L-Arg methyl ester recombinant enzyme Mus musculus
3.5.3.15 5.74
-
 benzoyl-L-Arg methyl ester native enzyme Mus musculus
3.5.3.15 10.7
-
 benzoyl-Arg ethyl ester recombinant enzyme Mus musculus
3.5.3.15 10.8
-
 benzoyl-arginine ethyl ester native enzyme Mus musculus