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Literature summary extracted from

  • Milstien, S.; Goldman, P.
    Metabolism of guanidinosuccinic acid. I. Characterization of a specific amidinohydrolase from Pseudomonas chlororaphis (1972), J. Biol. Chem., 247, 6280-6283.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.3.14 medicine test for N-amidino-L-aspartate in the urine of uremic patients Pseudomonas chlororaphis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.14 aspartic acid competitive inhibition Pseudomonas chlororaphis
3.5.3.14 Urea competitive inhibition Pseudomonas chlororaphis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.14 2.4
-
 N-amidino-L-aspartate HEPES buffer, pH 8, assay method B Pseudomonas chlororaphis
3.5.3.14 8.3
-
 N-amidino-L-aspartate HEPES buffer, pH 8, assay method A Pseudomonas chlororaphis
3.5.3.14 20
-
 N-amidino-L-aspartate phosphate buffer Pseudomonas chlororaphis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.14 Co2+
-
 Pseudomonas putida
3.5.3.14 Co2+ temperature-dependent activation Pseudomonas chlororaphis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.14 300000
-
 gel filtration Pseudomonas chlororaphis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.14 N-amidino-L-aspartic acid Pseudomonas putida
-
 L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-aspartic acid Pseudomonas chlororaphis
-
 L-aspartic acid + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.14 Pseudomonas chlororaphis
-
 fluorescent Pseudomonas
-
3.5.3.14 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.14
-
 Pseudomonas chlororaphis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.14 9.73
-
-
 Pseudomonas chlororaphis

Storage Stability

EC Number Storage Stability Organism
3.5.3.14 -15°C, stable for several months Pseudomonas chlororaphis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.14 guanidinoglutaric acid + H2O
-
 Pseudomonas chlororaphis ?
-
 ?
3.5.3.14 additional information
-
 Pseudomonas putida ?
-
 ?
3.5.3.14 additional information enzyme is specific for the L-isomer Pseudomonas chlororaphis ?
-
 ?
3.5.3.14 additional information N-amidino-D-aspartate, 3-guanidinopropionic acid, 4-guanidinobutyric acid, 2-amino-4-guanidinopropionic acid, agmatine, arginine, N-acetyl-arginine, creatinine, guanidinoacetic acid are no substrates Pseudomonas chlororaphis ?
-
 ?
3.5.3.14 N-amidino-L-aspartic acid
-
 Pseudomonas putida L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-aspartic acid
-
 Pseudomonas chlororaphis L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-aspartic acid + H2O
-
 Pseudomonas putida L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-aspartic acid + H2O
-
 Pseudomonas chlororaphis L-aspartic acid + urea
-
 ?
3.5.3.14 N-amidino-L-glutamate + H2O
-
 Pseudomonas putida L-glutamic acid + urea
-
 ?
3.5.3.14 N-amidino-L-glutamate + H2O
-
 Pseudomonas chlororaphis L-glutamic acid + urea
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.14 40 50 incubation at 40°C results in rapid activation, prolonged incubation causes irreversible loss of activity, incubation at 50°C 90% of the initial enzyme activity lost within 10 min Pseudomonas chlororaphis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.14 8
-
-
 Pseudomonas chlororaphis