BRENDA - Enzyme Database

Purification of monomeric agmatine iminohydrolase from soybean

Park, K.H.; Cho, Y.D.; Biochem. Biophys. Res. Commun. 174, 32-36 (1991)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
Co2+
-
Glycine max
3.5.3.12
Co2+
-
Zea mays
3.5.3.12
Cu2+
-
Glycine max
3.5.3.12
Cu2+
-
Zea mays
3.5.3.12
iodoacetamide
-
Glycine max
3.5.3.12
iodoacetamide
-
Zea mays
3.5.3.12
N-ethylmaleimide
-
Glycine max
3.5.3.12
N-ethylmaleimide
-
Zea mays
3.5.3.12
p-chloromercuribenzoate
-
Glycine max
3.5.3.12
p-chloromercuribenzoate
-
Zea mays
3.5.3.12
spermidine
-
Oryza sativa
3.5.3.12
spermine
-
Oryza sativa
3.5.3.12
Zn2+
-
Glycine max
3.5.3.12
Zn2+
-
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.19
-
agmatine
-
Zea mays
3.5.3.12
2.5
-
agmatine
-
Glycine max
3.5.3.12
15
-
agmatine
-
Oryza sativa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.3.12
cytosol
-
Glycine max
5829
-
3.5.3.12
cytosol
-
Zea mays
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.12
70000
-
gel filtration
Glycine max
3.5.3.12
85000
-
-
Zea mays
3.5.3.12
183000
-
-
Oryza sativa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Zea mays
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Glycine max
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Oryza sativa
-
N-carbamoylputrescine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.12
Glycine max
-
-
-
3.5.3.12
Oryza sativa
-
-
-
3.5.3.12
Zea mays
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.12
-
Glycine max
3.5.3.12
-
Oryza sativa
3.5.3.12
-
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.12
axis
-
Glycine max
-
3.5.3.12
seedling
-
Glycine max
-
3.5.3.12
seedling
-
Oryza sativa
-
3.5.3.12
seedling
-
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.3.12
172.2
-
-
Glycine max
Storage Stability
EC Number
Storage Stability
Organism
3.5.3.12
4C, purified enzyme lost 30% of the activity in 3 days
Zea mays
3.5.3.12
4C, purified enzyme lost 30% of the activity in 3 days
Glycine max
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
209404
Zea mays
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209404
Glycine max
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209404
Oryza sativa
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
additional information
arginine, creatine and citrulline are no substrates
209404
Zea mays
?
-
-
-
-
3.5.3.12
additional information
arginine, creatine and citrulline are no substrates
209404
Glycine max
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.12
dimer
-
Oryza sativa
3.5.3.12
dimer
-
Zea mays
3.5.3.12
monomer
1 * 70000, SDS-PAGE
Glycine max
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.3.12
28
-
-
Oryza sativa
3.5.3.12
50
-
-
Glycine max
3.5.3.12
60
-
-
Zea mays
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
6
-
-
Oryza sativa
3.5.3.12
6.5
-
-
Glycine max
3.5.3.12
6.5
-
-
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
Co2+
-
Glycine max
3.5.3.12
Co2+
-
Zea mays
3.5.3.12
Cu2+
-
Glycine max
3.5.3.12
Cu2+
-
Zea mays
3.5.3.12
iodoacetamide
-
Glycine max
3.5.3.12
iodoacetamide
-
Zea mays
3.5.3.12
N-ethylmaleimide
-
Glycine max
3.5.3.12
N-ethylmaleimide
-
Zea mays
3.5.3.12
p-chloromercuribenzoate
-
Glycine max
3.5.3.12
p-chloromercuribenzoate
-
Zea mays
3.5.3.12
spermidine
-
Oryza sativa
3.5.3.12
spermine
-
Oryza sativa
3.5.3.12
Zn2+
-
Glycine max
3.5.3.12
Zn2+
-
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.19
-
agmatine
-
Zea mays
3.5.3.12
2.5
-
agmatine
-
Glycine max
3.5.3.12
15
-
agmatine
-
Oryza sativa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.3.12
cytosol
-
Glycine max
5829
-
3.5.3.12
cytosol
-
Zea mays
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.12
70000
-
gel filtration
Glycine max
3.5.3.12
85000
-
-
Zea mays
3.5.3.12
183000
-
-
Oryza sativa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Zea mays
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Glycine max
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Oryza sativa
-
N-carbamoylputrescine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.12
-
Glycine max
3.5.3.12
-
Oryza sativa
3.5.3.12
-
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.12
axis
-
Glycine max
-
3.5.3.12
seedling
-
Glycine max
-
3.5.3.12
seedling
-
Oryza sativa
-
3.5.3.12
seedling
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.3.12
172.2
-
-
Glycine max
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.3.12
4C, purified enzyme lost 30% of the activity in 3 days
Zea mays
3.5.3.12
4C, purified enzyme lost 30% of the activity in 3 days
Glycine max
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
209404
Zea mays
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209404
Glycine max
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209404
Oryza sativa
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
additional information
arginine, creatine and citrulline are no substrates
209404
Zea mays
?
-
-
-
-
3.5.3.12
additional information
arginine, creatine and citrulline are no substrates
209404
Glycine max
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.12
dimer
-
Oryza sativa
3.5.3.12
dimer
-
Zea mays
3.5.3.12
monomer
1 * 70000, SDS-PAGE
Glycine max
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.3.12
28
-
-
Oryza sativa
3.5.3.12
50
-
-
Glycine max
3.5.3.12
60
-
-
Zea mays
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
6
-
-
Oryza sativa
3.5.3.12
6.5
-
-
Glycine max
3.5.3.12
6.5
-
-
Zea mays