BRENDA - Enzyme Database

Purification and characterization of putrescine synthase from cucumber seedlings. A multifunctional enzyme involved in putrescine biosynthesis

Prasad, G.L.; Adiga, P.R.; J. Biosci. 10, 373-391 (1986)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.5.3.12
highly unstable
Cucumis sativus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.3.6
15000
-
x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol
Cucumis sativus
2.1.3.6
18000
-
x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol
Cucumis sativus
2.1.3.6
44000
-
x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol
Cucumis sativus
2.1.3.6
150000
-
x * 150000, SDS-PAGE, nonreducing
Cucumis sativus
3.5.3.12
15000
-
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE
Cucumis sativus
3.5.3.12
44000
-
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE
Cucumis sativus
3.5.3.12
48000
-
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE
Cucumis sativus
3.5.3.12
66000
-
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE
Cucumis sativus
3.5.3.12
150000
-
gel filtration
Cucumis sativus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Lathyrus sativus
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Cucumis sativus
-
N-carbamoylputrescine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.3.6
Cucumis sativus
-
-
-
3.5.3.12
Cucumis sativus
-
cucumber
-
3.5.3.12
Lathyrus sativus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.12
-
Cucumis sativus
3.5.3.12
-
Lathyrus sativus
Reaction
EC Number
Reaction
Commentary
Organism
2.1.3.6
carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine
multifunctional enzyme that catalyzes the reactions of EC 2.1.3.3 ornithine carbamoyltransferase, EC 2.7.2.2 carbamate kinase and EC 3.5.3.12 agmatine deiminase, thus acting as putrescine synthase, converting agmatine and ornithine into putrescine and citrulline, respectively
Cucumis sativus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.3.6
seedling
-
Cucumis sativus
-
3.5.3.12
cotyledon
-
Cucumis sativus
-
3.5.3.12
seedling
-
Lathyrus sativus
-
3.5.3.12
seedling
-
Cucumis sativus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.3.12
2.64
-
-
Cucumis sativus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + ADP + phosphate
-
209403
Cucumis sativus
ATP + putrescine + NH3 + CO2
-
209403
Cucumis sativus
?
3.5.3.12
agmatine + H2O
-
209403
Lathyrus sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209403
Cucumis sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase
209403
Lathyrus sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase
209403
Cucumis sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + ornithine + H2O + phosphate
-
209403
Lathyrus sativus
putrescine + citrulline + NH3 + phosphate
-
209403
Lathyrus sativus
?
3.5.3.12
agmatine + ornithine + H2O + phosphate
-
209403
Cucumis sativus
putrescine + citrulline + NH3 + phosphate
-
209403
Cucumis sativus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.3.6
?
x * 150000, SDS-PAGE, nonreducing; x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol
Cucumis sativus
3.5.3.12
oligomer
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE
Cucumis sativus
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.3.12
highly unstable
Cucumis sativus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.3.6
15000
-
x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol
Cucumis sativus
2.1.3.6
18000
-
x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol
Cucumis sativus
2.1.3.6
44000
-
x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol
Cucumis sativus
2.1.3.6
150000
-
x * 150000, SDS-PAGE, nonreducing
Cucumis sativus
3.5.3.12
15000
-
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE
Cucumis sativus
3.5.3.12
44000
-
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE
Cucumis sativus
3.5.3.12
48000
-
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE
Cucumis sativus
3.5.3.12
66000
-
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE
Cucumis sativus
3.5.3.12
150000
-
gel filtration
Cucumis sativus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Lathyrus sativus
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Cucumis sativus
-
N-carbamoylputrescine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.12
-
Cucumis sativus
3.5.3.12
-
Lathyrus sativus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.3.6
seedling
-
Cucumis sativus
-
3.5.3.12
cotyledon
-
Cucumis sativus
-
3.5.3.12
seedling
-
Lathyrus sativus
-
3.5.3.12
seedling
-
Cucumis sativus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.3.12
2.64
-
-
Cucumis sativus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + ADP + phosphate
-
209403
Cucumis sativus
ATP + putrescine + NH3 + CO2
-
209403
Cucumis sativus
?
3.5.3.12
agmatine + H2O
-
209403
Lathyrus sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209403
Cucumis sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase
209403
Lathyrus sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase
209403
Cucumis sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + ornithine + H2O + phosphate
-
209403
Lathyrus sativus
putrescine + citrulline + NH3 + phosphate
-
209403
Lathyrus sativus
?
3.5.3.12
agmatine + ornithine + H2O + phosphate
-
209403
Cucumis sativus
putrescine + citrulline + NH3 + phosphate
-
209403
Cucumis sativus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.3.6
?
x * 150000, SDS-PAGE, nonreducing; x * 15000 + x * 18000 + x * 44000, SDS-PAGE, in the presence of 2-mercaptoethanol
Cucumis sativus
3.5.3.12
oligomer
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE
Cucumis sativus