BRENDA - Enzyme Database

Enzymic conversion of agmatine to putrescine in Lathyrus sativus seedlings. Purification and properties of a multifunctional enzyme (putrescine synthase)

Srivenugopal, K.S.; Adiga, P.R.; J. Biol. Chem. 256, 9532-9541 (1981)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.1.3.3
prolonged dialysis and freeze-thawing causes loss of activity
Lathyrus sativus
2.1.3.3
purified enzyme is highly unstable even in the presence of glycerol, dithiothreitol and Mg2+
Lathyrus sativus
2.1.3.6
highly unstable even in the presence of glycerol, dithiothreitol and Mg2+, activity is stabilized in dilute solutions, less than 0.05 mg protein/ml for about 3-4 h at 37°C by 0.25 mg/ml bovine serum albumine
Lathyrus sativus
2.7.2.2
all the component activities associated with putrescine synthase are stabilized in dilute solutions, 0.05 mg of protein per ml, for about 3-4 h at 37°C by 0.250 mg/ml bovine serum albumin
Lathyrus sativus
2.7.2.2
prolonged dialysis and freeze-thawing, loss of activity
Lathyrus sativus
2.7.2.2
purified enzyme is highly unstable even in presence of glycerol, dithiothreitol and Mg2+
Lathyrus sativus
3.5.3.12
highly unstable even in presence of glycerol, dithiothreitol and Mg2+, freeze-thawing and dialysis lead to considerable loss of activity
Lathyrus sativus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.3.3
cytoplasm
-
Lathyrus sativus
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.3.3
55000
-
1 * 55000, multifunctional enzyme, SDS-PAGE
Lathyrus sativus
2.1.3.3
56000
-
multifunctional enzyme with agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase activity, gel filtration
Lathyrus sativus
2.1.3.6
55000
-
1 * 55000, SDS-PAGE
Lathyrus sativus
2.1.3.6
56000
-
gel filtration
Lathyrus sativus
3.5.3.12
55000
-
gel filtration, PAGE
Lathyrus sativus
3.5.3.12
56000
-
gel filtration, Sephadex G-200
Lathyrus sativus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.2.2
ATP + NH3 + CO2
Lathyrus sativus
multifunctional enzyme, involved in biosynthesis of putrescine
ADP + carbamoyl phosphate
-
Lathyrus sativus
r
3.5.3.12
agmatine + H2O
Lathyrus sativus
-
N-carbamoylputrescine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.3.3
Lathyrus sativus
-
putrescine synthase has inherent activities of agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase
-
2.1.3.6
Lathyrus sativus
-
-
-
2.7.2.2
Lathyrus sativus
-
enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithine transcarbamylase and carbamate kinase
-
3.5.3.12
Lathyrus sativus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.3.3
putrescine synthase has inherent activities of agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase, ammonium sulfate, putrescine-Sepharose affinity column
Lathyrus sativus
2.1.3.6
MnCl2, ammonium sulfate, putrescine-CH-Sepharose affinity column
Lathyrus sativus
2.7.2.2
enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithine transcarbamylase and carbamate kinase
Lathyrus sativus
3.5.3.12
-
Lathyrus sativus
Reaction
EC Number
Reaction
Commentary
Organism
2.1.3.6
carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine
multifunctional enzyme that catalyzes the reactions of EC 2.1.3.3 ornithine carbamoyltransferase, EC 2.7.2.2 carbamate kinase and EC 3.5.3.12 agmatine deiminase, thus acting as putrescine synthase, converting agmatine and ornithine into putrescine and citrulline, respectively
Lathyrus sativus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.3.3
seedling
-
Lathyrus sativus
-
2.1.3.6
seedling
-
Lathyrus sativus
-
2.7.2.2
seedling
-
Lathyrus sativus
-
3.5.3.12
seedling
-
Lathyrus sativus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.3.3
0.47
-
multifunctional enzyme
Lathyrus sativus
3.5.3.12
1.1
-
-
Lathyrus sativus
Storage Stability
EC Number
Storage Stability
Organism
2.1.3.3
4°C, 48 h, complete loss of activity
Lathyrus sativus
2.1.3.6
4°C, 48 h, complete loss of activity
Lathyrus sativus
2.7.2.2
4°C, 48 h, complete loss of activity
Lathyrus sativus
3.5.3.12
4°C purified enzyme lost all component activities within 48 h
Lathyrus sativus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.3.3
carbamoyl phosphate + L-ornithine
enzyme also catalyzes the reactions of EC 2.1.3.6, EC 2.7.2.2 and EC 3.5.3.12, thus acting as putrescine synthase converting agmatine and ornithine into putrescine and citrulline
209399
Lathyrus sativus
phosphate + L-citrulline
-
209399
Lathyrus sativus
r
2.1.3.6
arsenate + N-carbamoylputrescine
-
209399
Lathyrus sativus
?
-
-
-
?
2.7.2.2
ADP + carbamoyl phosphate
-
209399
Lathyrus sativus
ATP + NH3 + CO2
-
-
-
r
2.7.2.2
ATP + NH3 + CO2
-
209399
Lathyrus sativus
ADP + carbamoyl phosphate
-
209399
Lathyrus sativus
r
2.7.2.2
ATP + NH3 + CO2
multifunctional enzyme, involved in biosynthesis of putrescine
209399
Lathyrus sativus
ADP + carbamoyl phosphate
-
209399
Lathyrus sativus
r
3.5.3.12
agmatine + H2O
-
209399
Lathyrus sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase
209399
Lathyrus sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + ornithine + H2O + phosphate
-
209399
Lathyrus sativus
putrescine + citrulline + NH3 + phosphate
-
209399
Lathyrus sativus
?
3.5.3.12
carbamoyl phosphate + ADP + H2O
-
209399
Lathyrus sativus
ATP + CO2 + NH3
-
209399
Lathyrus sativus
r
3.5.3.12
carbamoyl phosphate + ornithine
-
209399
Lathyrus sativus
citrulline + phosphate
-
209399
Lathyrus sativus
r
3.5.3.12
N-carbamoylputrescine + phosphate
-
209399
Lathyrus sativus
putrescine + carbamoyl phosphate
-
209399
Lathyrus sativus
r
Subunits
EC Number
Subunits
Commentary
Organism
2.1.3.3
monomer
1 * 55000, multifunctional enzyme, SDS-PAGE
Lathyrus sativus
2.1.3.6
monomer
1 * 55000, SDS-PAGE
Lathyrus sativus
3.5.3.12
monomer
1 * 55000, single polypeptide chain, SDS-PAGE
Lathyrus sativus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.3.3
8
-
-
Lathyrus sativus
3.5.3.12
7.5
-
putrescine transcarbamylase and carbamate kinase function
Lathyrus sativus
3.5.3.12
8
-
ornithine transcarbamylase function
Lathyrus sativus
3.5.3.12
8.8
-
agmatine iminohydrolase function
Lathyrus sativus
General Stability (protein specific)
EC Number
General Stability
Organism
2.1.3.3
prolonged dialysis and freeze-thawing causes loss of activity
Lathyrus sativus
2.1.3.3
purified enzyme is highly unstable even in the presence of glycerol, dithiothreitol and Mg2+
Lathyrus sativus
2.1.3.6
highly unstable even in the presence of glycerol, dithiothreitol and Mg2+, activity is stabilized in dilute solutions, less than 0.05 mg protein/ml for about 3-4 h at 37°C by 0.25 mg/ml bovine serum albumine
Lathyrus sativus
2.7.2.2
all the component activities associated with putrescine synthase are stabilized in dilute solutions, 0.05 mg of protein per ml, for about 3-4 h at 37°C by 0.250 mg/ml bovine serum albumin
Lathyrus sativus
2.7.2.2
prolonged dialysis and freeze-thawing, loss of activity
Lathyrus sativus
2.7.2.2
purified enzyme is highly unstable even in presence of glycerol, dithiothreitol and Mg2+
Lathyrus sativus
3.5.3.12
highly unstable even in presence of glycerol, dithiothreitol and Mg2+, freeze-thawing and dialysis lead to considerable loss of activity
Lathyrus sativus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.3.3
cytoplasm
-
Lathyrus sativus
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.3.3
55000
-
1 * 55000, multifunctional enzyme, SDS-PAGE
Lathyrus sativus
2.1.3.3
56000
-
multifunctional enzyme with agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase activity, gel filtration
Lathyrus sativus
2.1.3.6
55000
-
1 * 55000, SDS-PAGE
Lathyrus sativus
2.1.3.6
56000
-
gel filtration
Lathyrus sativus
3.5.3.12
55000
-
gel filtration, PAGE
Lathyrus sativus
3.5.3.12
56000
-
gel filtration, Sephadex G-200
Lathyrus sativus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.2.2
ATP + NH3 + CO2
Lathyrus sativus
multifunctional enzyme, involved in biosynthesis of putrescine
ADP + carbamoyl phosphate
-
Lathyrus sativus
r
3.5.3.12
agmatine + H2O
Lathyrus sativus
-
N-carbamoylputrescine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.3.3
putrescine synthase has inherent activities of agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase, ammonium sulfate, putrescine-Sepharose affinity column
Lathyrus sativus
2.1.3.6
MnCl2, ammonium sulfate, putrescine-CH-Sepharose affinity column
Lathyrus sativus
2.7.2.2
enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithine transcarbamylase and carbamate kinase
Lathyrus sativus
3.5.3.12
-
Lathyrus sativus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.3.3
seedling
-
Lathyrus sativus
-
2.1.3.6
seedling
-
Lathyrus sativus
-
2.7.2.2
seedling
-
Lathyrus sativus
-
3.5.3.12
seedling
-
Lathyrus sativus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.3.3
0.47
-
multifunctional enzyme
Lathyrus sativus
3.5.3.12
1.1
-
-
Lathyrus sativus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.1.3.3
4°C, 48 h, complete loss of activity
Lathyrus sativus
2.1.3.6
4°C, 48 h, complete loss of activity
Lathyrus sativus
2.7.2.2
4°C, 48 h, complete loss of activity
Lathyrus sativus
3.5.3.12
4°C purified enzyme lost all component activities within 48 h
Lathyrus sativus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.3.3
carbamoyl phosphate + L-ornithine
enzyme also catalyzes the reactions of EC 2.1.3.6, EC 2.7.2.2 and EC 3.5.3.12, thus acting as putrescine synthase converting agmatine and ornithine into putrescine and citrulline
209399
Lathyrus sativus
phosphate + L-citrulline
-
209399
Lathyrus sativus
r
2.1.3.6
arsenate + N-carbamoylputrescine
-
209399
Lathyrus sativus
?
-
-
-
?
2.7.2.2
ADP + carbamoyl phosphate
-
209399
Lathyrus sativus
ATP + NH3 + CO2
-
-
-
r
2.7.2.2
ATP + NH3 + CO2
-
209399
Lathyrus sativus
ADP + carbamoyl phosphate
-
209399
Lathyrus sativus
r
2.7.2.2
ATP + NH3 + CO2
multifunctional enzyme, involved in biosynthesis of putrescine
209399
Lathyrus sativus
ADP + carbamoyl phosphate
-
209399
Lathyrus sativus
r
3.5.3.12
agmatine + H2O
-
209399
Lathyrus sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase
209399
Lathyrus sativus
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + ornithine + H2O + phosphate
-
209399
Lathyrus sativus
putrescine + citrulline + NH3 + phosphate
-
209399
Lathyrus sativus
?
3.5.3.12
carbamoyl phosphate + ADP + H2O
-
209399
Lathyrus sativus
ATP + CO2 + NH3
-
209399
Lathyrus sativus
r
3.5.3.12
carbamoyl phosphate + ornithine
-
209399
Lathyrus sativus
citrulline + phosphate
-
209399
Lathyrus sativus
r
3.5.3.12
N-carbamoylputrescine + phosphate
-
209399
Lathyrus sativus
putrescine + carbamoyl phosphate
-
209399
Lathyrus sativus
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.3.3
monomer
1 * 55000, multifunctional enzyme, SDS-PAGE
Lathyrus sativus
2.1.3.6
monomer
1 * 55000, SDS-PAGE
Lathyrus sativus
3.5.3.12
monomer
1 * 55000, single polypeptide chain, SDS-PAGE
Lathyrus sativus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.3.3
8
-
-
Lathyrus sativus
3.5.3.12
7.5
-
putrescine transcarbamylase and carbamate kinase function
Lathyrus sativus
3.5.3.12
8
-
ornithine transcarbamylase function
Lathyrus sativus
3.5.3.12
8.8
-
agmatine iminohydrolase function
Lathyrus sativus