BRENDA - Enzyme Database

Corn agmatine iminohydrolase. Purification and properties

Yanagisawa, H.; Suzuki, Y.; Plant Physiol. 67, 697-700 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
Ca2+
-
Zea mays
3.5.3.12
cadaverine
-
Arachis hypogaea
3.5.3.12
Co2+
1 mM 55% inhibition
Zea mays
3.5.3.12
Cu2+
-
Arachis hypogaea
3.5.3.12
Cu2+
1 mM 100% inhibition
Zea mays
3.5.3.12
Fe2+
-
Zea mays
3.5.3.12
Mg2+
-
Zea mays
3.5.3.12
N,N'-diguanidinobutane
-
Zea mays
3.5.3.12
Ni2+
-
Zea mays
3.5.3.12
p-chloromercuribenzoate
-
Arachis hypogaea
3.5.3.12
p-chloromercuribenzoate
-
Zea mays
3.5.3.12
putrescine
-
Arachis hypogaea
3.5.3.12
spermidine
-
Arachis hypogaea
3.5.3.12
spermine
-
Arachis hypogaea
3.5.3.12
tryptamine
-
Arachis hypogaea
3.5.3.12
Zn2+
-
Arachis hypogaea
3.5.3.12
Zn2+
1 mM 85% inhibition
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.19
-
agmatine
-
Zea mays
3.5.3.12
0.76
-
agmatine
-
Arachis hypogaea
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.3.12
cytosol
-
Zea mays
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.12
43000
-
2 * 43000, SDS-PAGE
Zea mays
3.5.3.12
85000
-
gel filtration
Zea mays
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Zea mays
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Arachis hypogaea
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Helianthus annuus
-
N-carbamoylputrescine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.12
Arachis hypogaea
-
-
-
3.5.3.12
Helianthus annuus
-
-
-
3.5.3.12
Zea mays
-
L. cv Goldencross Bantam T51
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.12
-
Arachis hypogaea
3.5.3.12
-
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.12
cotyledon
-
Arachis hypogaea
-
3.5.3.12
seedling
-
Helianthus annuus
-
3.5.3.12
seedling
-
Zea mays
-
3.5.3.12
shoot
-
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.12
876.3
-
-
Zea mays
Storage Stability
EC Number
Storage Stability
Organism
3.5.3.12
4°C, purified enzyme lost 30% of the activity in 3 days
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
209398
Zea mays
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209398
Arachis hypogaea
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209398
Helianthus annuus
N-carbamoylputrescine + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.12
dimer
2 * 43000, SDS-PAGE
Zea mays
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.3.12
60
-
-
Zea mays
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.3.12
50
-
exposure for 10 min, decrease of 50% in the activity
Zea mays
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
6.5
-
-
Zea mays
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.12
6
7
activity rapidly declining below pH 6.0 and declining less rapidly above pH 7.0
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
Ca2+
-
Zea mays
3.5.3.12
cadaverine
-
Arachis hypogaea
3.5.3.12
Co2+
1 mM 55% inhibition
Zea mays
3.5.3.12
Cu2+
-
Arachis hypogaea
3.5.3.12
Cu2+
1 mM 100% inhibition
Zea mays
3.5.3.12
Fe2+
-
Zea mays
3.5.3.12
Mg2+
-
Zea mays
3.5.3.12
N,N'-diguanidinobutane
-
Zea mays
3.5.3.12
Ni2+
-
Zea mays
3.5.3.12
p-chloromercuribenzoate
-
Arachis hypogaea
3.5.3.12
p-chloromercuribenzoate
-
Zea mays
3.5.3.12
putrescine
-
Arachis hypogaea
3.5.3.12
spermidine
-
Arachis hypogaea
3.5.3.12
spermine
-
Arachis hypogaea
3.5.3.12
tryptamine
-
Arachis hypogaea
3.5.3.12
Zn2+
-
Arachis hypogaea
3.5.3.12
Zn2+
1 mM 85% inhibition
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.19
-
agmatine
-
Zea mays
3.5.3.12
0.76
-
agmatine
-
Arachis hypogaea
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.3.12
cytosol
-
Zea mays
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.12
43000
-
2 * 43000, SDS-PAGE
Zea mays
3.5.3.12
85000
-
gel filtration
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Zea mays
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Arachis hypogaea
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Helianthus annuus
-
N-carbamoylputrescine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.12
-
Arachis hypogaea
3.5.3.12
-
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.12
cotyledon
-
Arachis hypogaea
-
3.5.3.12
seedling
-
Helianthus annuus
-
3.5.3.12
seedling
-
Zea mays
-
3.5.3.12
shoot
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.12
876.3
-
-
Zea mays
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.3.12
4°C, purified enzyme lost 30% of the activity in 3 days
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
209398
Zea mays
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209398
Arachis hypogaea
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209398
Helianthus annuus
N-carbamoylputrescine + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.12
dimer
2 * 43000, SDS-PAGE
Zea mays
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.3.12
60
-
-
Zea mays
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.3.12
50
-
exposure for 10 min, decrease of 50% in the activity
Zea mays
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
6.5
-
-
Zea mays
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.12
6
7
activity rapidly declining below pH 6.0 and declining less rapidly above pH 7.0
Zea mays