BRENDA - Enzyme Database

Purification and properties of agmatine iminohydrolase from groundnut cotyledons

Sindhu, R.K.; Desai, H.V.; Phytochemistry 18, 1937-1938 (1979)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
cadaverine
competitive inhibition
Arachis hypogaea
3.5.3.12
Cu2+
-
Arachis hypogaea
3.5.3.12
Cu2+
-
Zea mays
3.5.3.12
Fe3+
-
Arachis hypogaea
3.5.3.12
Hg2+
-
Arachis hypogaea
3.5.3.12
p-chloromercuribenzoate
-
Arachis hypogaea
3.5.3.12
p-chloromercuribenzoate
-
Zea mays
3.5.3.12
putrescine
-
Arachis hypogaea
3.5.3.12
spermidine
competitive inhibition
Arachis hypogaea
3.5.3.12
spermine
10 mM, 50% inhibition
Arachis hypogaea
3.5.3.12
tryptamine
2.5 mM, 50% inhibition
Arachis hypogaea
3.5.3.12
Zn2+
-
Arachis hypogaea
3.5.3.12
Zn2+
-
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.76
-
agmatine
-
Arachis hypogaea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Escherichia coli
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Zea mays
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Arachis hypogaea
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Helianthus annuus
-
N-carbamoylputrescine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.12
Arachis hypogaea
-
groundnut, L. cv Punjab-1
-
3.5.3.12
Escherichia coli
-
-
-
3.5.3.12
Helianthus annuus
-
-
-
3.5.3.12
Zea mays
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.12
-
Arachis hypogaea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.12
cotyledon
-
Arachis hypogaea
-
3.5.3.12
leaf
-
Arachis hypogaea
-
3.5.3.12
leaf
-
Zea mays
-
3.5.3.12
seed
-
Zea mays
-
3.5.3.12
seedling
-
Helianthus annuus
-
3.5.3.12
seedling
-
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.3.12
0.135
-
-
Arachis hypogaea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
209397
Escherichia coli
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209397
Zea mays
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209397
Arachis hypogaea
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209397
Helianthus annuus
N-carbamoylputrescine + NH3
-
-
-
?
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.5.3.12
55
-
retains 44% activity when exposed for 10 min, completely inactivated at 60░C under the same conditions
Arachis hypogaea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
5.5
8.5
-
Arachis hypogaea
3.5.3.12
6.5
7.5
-
Zea mays
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.12
2.5
-
tryptamine
-
Arachis hypogaea
3.5.3.12
10
-
spermine
-
Arachis hypogaea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
cadaverine
competitive inhibition
Arachis hypogaea
3.5.3.12
Cu2+
-
Arachis hypogaea
3.5.3.12
Cu2+
-
Zea mays
3.5.3.12
Fe3+
-
Arachis hypogaea
3.5.3.12
Hg2+
-
Arachis hypogaea
3.5.3.12
p-chloromercuribenzoate
-
Arachis hypogaea
3.5.3.12
p-chloromercuribenzoate
-
Zea mays
3.5.3.12
putrescine
-
Arachis hypogaea
3.5.3.12
spermidine
competitive inhibition
Arachis hypogaea
3.5.3.12
spermine
10 mM, 50% inhibition
Arachis hypogaea
3.5.3.12
tryptamine
2.5 mM, 50% inhibition
Arachis hypogaea
3.5.3.12
Zn2+
-
Arachis hypogaea
3.5.3.12
Zn2+
-
Zea mays
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.12
2.5
-
tryptamine
-
Arachis hypogaea
3.5.3.12
10
-
spermine
-
Arachis hypogaea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.76
-
agmatine
-
Arachis hypogaea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Escherichia coli
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Zea mays
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Arachis hypogaea
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Helianthus annuus
-
N-carbamoylputrescine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.12
-
Arachis hypogaea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.12
cotyledon
-
Arachis hypogaea
-
3.5.3.12
leaf
-
Arachis hypogaea
-
3.5.3.12
leaf
-
Zea mays
-
3.5.3.12
seed
-
Zea mays
-
3.5.3.12
seedling
-
Helianthus annuus
-
3.5.3.12
seedling
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.3.12
0.135
-
-
Arachis hypogaea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
209397
Escherichia coli
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209397
Zea mays
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209397
Arachis hypogaea
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
209397
Helianthus annuus
N-carbamoylputrescine + NH3
-
-
-
?
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.5.3.12
55
-
retains 44% activity when exposed for 10 min, completely inactivated at 60░C under the same conditions
Arachis hypogaea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
5.5
8.5
-
Arachis hypogaea
3.5.3.12
6.5
7.5
-
Zea mays