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Literature summary extracted from

  • Van Der Werf, P.; Orlowski, M.; Meister, A.
    Enzymatic conversion of 5-oxo-L-proline (L-pyrrolidone carboxylate) to L-glutamate coupled with cleavage of adenosine triphosphate to adenosine diphosphate, a reaction in the gamma-glutamyl cycle (1971), Proc. Natl. Acad. Sci. USA, 68, 2982-2985.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.9 0.1
-
 MgATP2-
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.9 K+ requires monovalent cation, K+ or NH4+ Rattus norvegicus
3.5.2.9 Mg2+
-
 Rattus norvegicus
3.5.2.9 Mn2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.9 5-oxo-L-proline + H2O + ATP Homo sapiens
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Rattus norvegicus
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Sus scrofa
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Ovis aries
-
 L-glutamate + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.9 Homo sapiens
-
 human
-
3.5.2.9 Ovis aries
-
 sheep
-
3.5.2.9 Rattus norvegicus
-
 rat
-
3.5.2.9 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.9
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.9 brain
-
 Homo sapiens
-
3.5.2.9 brain
-
 Rattus norvegicus
-
3.5.2.9 brain
-
 Sus scrofa
-
3.5.2.9 cardiac muscle
-
 Rattus norvegicus
-
3.5.2.9 cardiac muscle
-
 Sus scrofa
-
3.5.2.9 intestine
-
 Rattus norvegicus
-
3.5.2.9 kidney
-
 Homo sapiens
-
3.5.2.9 kidney
-
 Rattus norvegicus
-
3.5.2.9 kidney
-
 Sus scrofa
-
3.5.2.9 kidney
-
 Ovis aries
-
3.5.2.9 liver
-
 Homo sapiens
-
3.5.2.9 liver
-
 Rattus norvegicus
-
3.5.2.9 liver
-
 Sus scrofa
-
3.5.2.9 lung
-
 Homo sapiens
-
3.5.2.9 lung
-
 Rattus norvegicus
-
3.5.2.9 spleen
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.5.2.9 4°C, loses progressively activity, 60% initial activity lost in 6 days, activity completely restored upon addition of 5 mM dithiothreitol Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Homo sapiens L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Rattus norvegicus L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Sus scrofa L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Ovis aries L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 MgATP2- + H2O
-
 Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.9 7.8
-
-
 Rattus norvegicus