Literature summary extracted from

Ellerby, L.M.; Escobar, W.A.; Fink, A.L.; Mitchinson, C.; Wells, J.A.
The role of lysine-234 in beta-lactamase catalysis probed by site directed mutagenesis (1990), Biochemistry, 29, 5797-5806.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.5.2.6	expressed in Bacillus subtilis	Bacillus licheniformis

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.5.2.6	K234A	no significant structural change, mutant with decreased Km 1-2 orders of magnitude and kcat 2-3 orders of magnitude	Bacillus licheniformis
3.5.2.6	K234E	no significant structural change, mutant with decreased Km 1-2 orders of magnitude and kcat 2-3 orders of magnitude	Bacillus licheniformis

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.5.2.6	0.041	-	nitrocefin	wild type	Bacillus licheniformis
3.5.2.6	0.1	-	phenoxymethylpenicillin	wild type	Bacillus licheniformis
3.5.2.6	0.12	-	benzylpenicillin	wild type	Bacillus licheniformis
3.5.2.6	0.14	-	7-(thienyl-2-acetamido)-3-[2-(4-,N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid	wild type	Bacillus licheniformis
3.5.2.6	0.21	-	ampicillin	wild type	Bacillus licheniformis

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.5.2.6	extracellular	-	Bacillus licheniformis	-	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.2.6	Bacillus licheniformis	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.2.6	-	Bacillus licheniformis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.2.6	7-(thienyl-2-acetamido)-3-[2-(4-N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid + H2O	i.e. PADAC	Bacillus licheniformis	(2R)-2-[(R)-carboxy{(E)-[1-hydroxy-2-(thiophen-2-yl)ethylidene]amino}methyl]-5-{[(2E)-2-{[4-(dimethyliminio)cyclohexa-2,5-dien-1-ylidene]hydrazinylidene}pyridin-1(2H)-yl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylate	-	?
3.5.2.6	ampicillin + H2O	-	Bacillus licheniformis	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
3.5.2.6	benzylpenicillin + H2O	-	Bacillus licheniformis	(2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
3.5.2.6	nitrocefin + H2O	-	Bacillus licheniformis	(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
3.5.2.6	phenoxymethylpenicillin + H2O	-	Bacillus licheniformis	(2R,4S)-2-[(R)-carboxy(2-phenoxyacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
3.5.2.6	50	75	pH 7: stable up to 58, 62, 68°C for K234E, wild type and K234A respectively	Bacillus licheniformis

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.5.2.6	667	-	7-(thienyl-2-acetamido)-3-[2-(4-,N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid	-	Bacillus licheniformis
3.5.2.6	1090	-	nitrocefin	-	Bacillus licheniformis
3.5.2.6	2240	-	ampicillin	-	Bacillus licheniformis
3.5.2.6	2550	-	phenoxymethylpenicillin	-	Bacillus licheniformis
3.5.2.6	2650	-	benzylpenicillin	-	Bacillus licheniformis

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.5.2.6	6.5	-	substrate phenoxymethylpenicillin	Bacillus licheniformis

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
3.5.2.6	4	9	substrate phenoxymethylpenicillin: kcat 1/6 at pH 9 compared to pH 6.5	Bacillus licheniformis

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Release 2023.1 (January 2023)