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Literature summary extracted from

  • Thornewell, J.; Waley, G.
    Site-directed mutagenesis and substrate-induced inactivation of beta-lactamase I (1992), Biochem. J., 288, 1045-1051.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Bacillus subtilis Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 A123C stability towards cloxacillin not enhanced Bacillus cereus
3.5.2.6 A77C stability towards cloxacillin not enhanced Bacillus cereus
3.5.2.6 DELTA1-17 mutant lacking the first 17 residues, first alpha helix, reduced activity, no hydrolysis of cloxacillin Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 Cloxacillin hydrolysis or inhibition, depending on pH, temperature, ratio enzyme/cloaxacillin Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.055
-
 nitrocefin
-
 Bacillus cereus
3.5.2.6 0.065
-
 benzylpenicillin
-
 Bacillus cereus
3.5.2.6 0.22
-
 cephalosporin C
-
 Bacillus cereus
3.5.2.6 0.27
-
 Cloxacillin pH 7, 20°C Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.2.6 extracellular
-
 Bacillus subtilis
-
-
3.5.2.6 extracellular
-
 Bacillus cereus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Bacillus cereus
-
-
-
3.5.2.6 Bacillus cereus 569/H/9
-
-
-
3.5.2.6 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 benzylpenicillin + H2O
-
 Bacillus cereus (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Bacillus cereus 569/H/9 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cephalosporin C + H2O poor substrate, beta-lactamse I Bacillus cereus N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
3.5.2.6 cephalosporin C + H2O poor substrate, beta-lactamse I Bacillus cereus 569/H/9 N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
3.5.2.6 cloxacillin + H2O hydrolysis or inhibition, depending on pH, temperature, ratio enzyme/cloaxcillin Bacillus cereus (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cloxacillin + H2O hydrolysis or inhibition, depending on pH, temperature, ratio enzyme/cloaxcillin Bacillus cereus 569/H/9 (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Bacillus cereus (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Bacillus cereus 569/H/9 (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.2
-
 cephalosporin C
-
 Bacillus cereus
3.5.2.6 34
-
 nitrocefin
-
 Bacillus cereus
3.5.2.6 2200
-
 benzylpenicillin
-
 Bacillus cereus