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Literature summary extracted from

  • Ledent, P.; Raquet, X.; Joris, B.; Van Beeumen, J.; Frere, J.M.
    A comparative study of beta-lactamases (1993), Biochem. J., 292, 439-443.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 subcloning Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 6-beta-iodopenicillanic acid enzyme 2 OXA2 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 24600
-
 enzyme 1, OXA1, gel filtration Escherichia coli
3.5.2.6 35000
-
 enzyme 3, PSE2, gel fitration Escherichia coli
3.5.2.6 50600
-
 enzyme 2, OXA2, gel fitration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Escherichia coli
-
-
-
3.5.2.6 Escherichia coli JM105
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.6 13
-
 enzyme OXA1 Escherichia coli
3.5.2.6 41
-
 enzyme PSE2 Escherichia coli
3.5.2.6 250
-
 enzyme OXA2 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 benzylpenicillin + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Escherichia coli JM105 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefamandole + H2O
-
 Escherichia coli (2R)-2-[(R)-carboxy{[(2R)-2-hydroxy-2-phenylacetyl]amino}methyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefazolin + H2O
-
 Escherichia coli (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloglycin + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-{[(2R)-2-amino-2-phenylacetyl]amino}(carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloglycin + H2O
-
 Escherichia coli JM105 (2R)-5-[(acetyloxy)methyl]-2-[(R)-{[(2R)-2-amino-2-phenylacetyl]amino}(carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Escherichia coli (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Escherichia coli JM105 (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalosporin C + H2O
-
 Escherichia coli N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
3.5.2.6 cephalosporin C + H2O
-
 Escherichia coli JM105 N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
3.5.2.6 cloxacillin + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cloxacillin + H2O
-
 Escherichia coli JM105 (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Escherichia coli (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 monomer 1* 35000 Escherichia coli