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Literature summary extracted from

  • Viadiu, H.; Osuna, J.; Fink, A.L.; Soberon, X.
    A new TEM beta-lactamase double mutant with broadened specificity reveals substrate-dependent functional interactions (1995), J. Biol. Chem., 270, 781-787.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 E104K E104M increased kcat Escherichia coli
3.5.2.6 E104K/G238S
-
Escherichia coli
3.5.2.6 E104M/G238S E104M/G238S 1000 fold higher hydrolysis of cefotaxime Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.033
-
benzylpenicillin
-
Escherichia coli
3.5.2.6 0.148
-
ampicillin
-
Escherichia coli
3.5.2.6 0.242
-
cefuroxime
-
Escherichia coli
3.5.2.6 0.35
-
6-aminopenicillanic acid
-
Escherichia coli
3.5.2.6 0.6
-
cephaloridine
-
Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Escherichia coli
-
-
-
3.5.2.6 Escherichia coli JM101
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 6-aminopenicillanic acid + H2O
-
Escherichia coli (2R,4S)-2-[amino(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 6-aminopenicillanic acid + H2O
-
Escherichia coli JM101 (2R,4S)-2-[amino(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 ampicillin + H2O
-
Escherichia coli (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 ampicillin + H2O
-
Escherichia coli JM101 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 benzylpenicillin + H2O
-
Escherichia coli (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 benzylpenicillin + H2O
-
Escherichia coli JM101 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 cefuroxime + H2O
-
Escherichia coli (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 cefuroxime + H2O
-
Escherichia coli JM101 (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 cephaloridine + H2O
-
Escherichia coli (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
3.5.2.6 cephaloridine + H2O
-
Escherichia coli JM101 (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 1
-
cefuroxime
-
Escherichia coli
3.5.2.6 570
-
cephaloridine
-
Escherichia coli
3.5.2.6 659
-
6-aminopenicillanic acid
-
Escherichia coli
3.5.2.6 782
-
benzylpenicillin
-
Escherichia coli
3.5.2.6 1090
-
ampicillin
-
Escherichia coli