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Literature summary extracted from

  • Walsh, T.R.; Gamblin, S.; Emery, D.; McGowan, A.P.; Bennett, P.M.
    Enzyme kinetics and biochemical analysis of ImiS, the metallo-beta-lactamase from Aeromonas sobria 163a (1996), J. Antimicrob. Chemother., 37, 423-431.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 cefoxitin 40% loss of activity Aeromonas sobria
3.5.2.6 EDTA
-
 Aeromonas sobria
3.5.2.6 latamoxef 45% loss of activity Aeromonas sobria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.016
-
 nitrocefin
-
 Aeromonas sobria
3.5.2.6 0.18
-
 Imipenem
-
 Aeromonas sobria
3.5.2.6 0.25
-
 benzylpenicillin
-
 Aeromonas sobria
3.5.2.6 0.26
-
 carbenicillin
-
 Aeromonas sobria
3.5.2.6 0.33
-
 meropenem
-
 Aeromonas sobria
3.5.2.6 0.51
-
 ampicillin
-
 Aeromonas sobria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 28000
-
 SDS-PAGE Aeromonas sobria

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Aeromonas sobria
-
-
-
3.5.2.6 Aeromonas sobria 163a
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Aeromonas sobria

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.6 110
-
-
 Aeromonas sobria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Aeromonas sobria (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Aeromonas sobria 163a (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Aeromonas sobria (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Aeromonas sobria 163a (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Aeromonas sobria (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Aeromonas sobria 163a (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Aeromonas sobria (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Aeromonas sobria 163a (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Aeromonas sobria (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Aeromonas sobria 163a (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Aeromonas sobria (2R)-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.0667
-
 nitrocefin
-
 Aeromonas sobria
3.5.2.6 0.5
-
 carbenicillin
-
 Aeromonas sobria
3.5.2.6 0.533
-
 benzylpenicillin
-
 Aeromonas sobria
3.5.2.6 1.1
-
 ampicillin
-
 Aeromonas sobria
3.5.2.6 160
-
 Imipenem
-
 Aeromonas sobria
3.5.2.6 1100
-
 meropenem
-
 Aeromonas sobria