Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Toney, J.H.; Wu, J.K.; Overbye, K.M.; Thompson, C.M.; Pompliano, D.L.
    High-yield expression, purification, and characterization of active, soluble Bacteroides fragilis metallo-beta-lactamase, CcrA (1997), Protein Expr. Purif., 9, 355-362.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli Bacteroides fragilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 CdCl2
-
 Bacteroides fragilis
3.5.2.6 ZnCl2
-
 Bacteroides fragilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.003
-
 cephaloridine
-
 Bacteroides fragilis
3.5.2.6 0.009
-
 cefotaxime
-
 Bacteroides fragilis
3.5.2.6 0.026
-
 nitrocefin
-
 Bacteroides fragilis
3.5.2.6 0.054
-
 cefoxitin
-
 Bacteroides fragilis
3.5.2.6 0.097
-
 penicillin G
-
 Bacteroides fragilis
3.5.2.6 0.13
-
 piperacillin
-
 Bacteroides fragilis
3.5.2.6 0.13
-
 carbenicillin
-
 Bacteroides fragilis
3.5.2.6 0.2
-
 Imipenem
-
 Bacteroides fragilis
3.5.2.6 0.25
-
 ampicillin
-
 Bacteroides fragilis
3.5.2.6 0.34
-
 ceftazidime
-
 Bacteroides fragilis
3.5.2.6 1.7
-
 Methicillin
-
 Bacteroides fragilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 25360
-
 mass spectrometry Bacteroides fragilis

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Bacteroides fragilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Bacteroides fragilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.6 30
-
 nitrocefin Bacteroides fragilis
3.5.2.6 240
-
 nitrocefin, non-fusion protein Bacteroides fragilis
3.5.2.6 260
-
 benzylpenicillin Bacteroides fragilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Bacteroides fragilis (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Bacteroides fragilis (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Bacteroides fragilis (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Bacteroides fragilis (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Bacteroides fragilis (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Bacteroides fragilis (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 imipenem + H2O
-
 Bacteroides fragilis (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 methicillin + H2O
-
 Bacteroides fragilis (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Bacteroides fragilis (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 penicillin G + H2O
-
 Bacteroides fragilis (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Bacteroides fragilis (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 monomer 1* 25357 Bacteroides fragilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 20
-
 cephaloridine
-
 Bacteroides fragilis
3.5.2.6 25
-
 cefotaxime
-
 Bacteroides fragilis
3.5.2.6 75
-
 ampicillin
-
 Bacteroides fragilis
3.5.2.6 100
-
 Imipenem
-
 Bacteroides fragilis
3.5.2.6 100
-
 piperacillin
-
 Bacteroides fragilis
3.5.2.6 110
-
 penicillin G
-
 Bacteroides fragilis
3.5.2.6 140
-
 ceftazidime
-
 Bacteroides fragilis
3.5.2.6 150
-
 cefoxitin
-
 Bacteroides fragilis
3.5.2.6 200
-
 nitrocefin
-
 Bacteroides fragilis
3.5.2.6 260
-
 carbenicillin
-
 Bacteroides fragilis
3.5.2.6 350
-
 Methicillin
-
 Bacteroides fragilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.6 5.5 7.5
-
 Bacteroides fragilis