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Literature summary extracted from

  • Guo, F.; Huynh, J.; Dmitrienko, G.I.; Viswanatha, T.; Clarke, A.J.
    The role of the non-conserved residue at position 104 of class A beta-lactamases in susceptibility to mechanism-based inhibitors (1999), Biochim. Biophys. Acta, 1431, 132-147.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 D104A km for benzylpenicillin and methicillin increased 2 fold, no change in catalytic activity Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 6-beta-trifluoromethanesulfonamidopenicillanic acid
-
 Bacillus cereus
3.5.2.6 6-bromopenicillanic acid
-
 Bacillus cereus
3.5.2.6 clavulanic acid
-
 Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.2.6 extracellular
-
 Staphylococcus aureus
-
-
3.5.2.6 extracellular
-
 Bacillus cereus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.6 ampicillin + H2O Bacillus cereus
-
 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O Bacillus cereus 569/H/9
-
 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Bacillus cereus
-
-
-
3.5.2.6 Bacillus cereus 569/H/9
-
-
-
3.5.2.6 Staphylococcus aureus
-
-
-
3.5.2.6 Staphylococcus aureus PC1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Staphylococcus aureus
3.5.2.6
-
 Bacillus cereus

Storage Stability

EC Number Storage Stability Organism
3.5.2.6 -20°C, 50 mM sodium phosphate buffer, pH 6.8, 50% glycerol Staphylococcus aureus
3.5.2.6 -20°C, 50 mM sodium phosphate buffer, pH 6.8, 50% glycerol Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Staphylococcus aureus (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Bacillus cereus (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Bacillus cereus 569/H/9 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Staphylococcus aureus PC1 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Staphylococcus aureus (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Bacillus cereus (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Bacillus cereus 569/H/9 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Staphylococcus aureus PC1 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 methicillin + H2O
-
 Bacillus cereus (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 methicillin + H2O
-
 Bacillus cereus 569/H/9 (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Staphylococcus aureus (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Bacillus cereus (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Bacillus cereus 569/H/9 (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Staphylococcus aureus PC1 (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 beta-lactamse A-D A,C and D enzymes utilize a nucleophilic serine at the active center which becomes acylated by substrate, B enzymes are metalloenzymes requiring Zn2+ for activity Staphylococcus aureus
3.5.2.6 beta-lactamse A-D A,C and D enzymes utilize a nucleophilic serine at the active center which becomes acylated by substrate, B enzymes are metalloenzymes requiring Zn2+ for activity Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 8
-
 Oxacillin
-
 Staphylococcus aureus
3.5.2.6 135
-
 benzylpenicillin
-
 Staphylococcus aureus
3.5.2.6 145
-
 Methicillin
-
 Bacillus cereus
3.5.2.6 261
-
 Oxacillin
-
 Bacillus cereus
3.5.2.6 550
-
 ampicillin
-
 Staphylococcus aureus
3.5.2.6 1350
-
 benzylpenicillin
-
 Bacillus cereus
3.5.2.6 5400
-
 ampicillin
-
 Bacillus cereus