Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vakulenko, S.B.; Taibi-Tronche, P.; Toth, M.; Massova, I.; Lerner, S.A.; Mobashery, S.
    Effects on substrate profile by mutational substitutions at position 164 and 179 of the class A TEMpUC19beta-lactamase from Escherichia coli (1999), J. Biol. Chem., 274, 23052-23060.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.6
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 D179G activity unchanged or enhanced Escherichia coli
3.5.2.6 D179N activity unchanged or enhanced Escherichia coli
3.5.2.6 R164N activity unchanged or enhanced Escherichia coli
3.5.2.6 R164S activity unchanged or enhanced Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.027
-
 ampicillin
-
 Escherichia coli
3.5.2.6 0.07
-
 piperacillin
-
 Escherichia coli
3.5.2.6 0.12
-
 penicillin G
-
 Escherichia coli
3.5.2.6 0.2
-
 ceftazidime
-
 Escherichia coli
3.5.2.6 0.21
-
 cephalothin
-
 Escherichia coli
3.5.2.6 0.23
-
 cefotaxime
-
 Escherichia coli
3.5.2.6 0.24
-
 cefepime
-
 Escherichia coli
3.5.2.6 0.55
-
 aztreonam
-
 Escherichia coli
3.5.2.6 0.67
-
 cephaloridine
-
 Escherichia coli
3.5.2.6 0.95
-
 cephalosporin C
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Escherichia coli
-
-
-
3.5.2.6 Escherichia coli JM83
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Escherichia coli JM83 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 aztreonam + H2O
-
 Escherichia coli [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
3.5.2.6 aztreonam + H2O
-
 Escherichia coli JM83 [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
3.5.2.6 cefepime + H2O
-
 Escherichia coli (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Escherichia coli (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Escherichia coli (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Escherichia coli JM83 (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalosporin C + H2O
-
 Escherichia coli N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
3.5.2.6 cephalosporin C + H2O
-
 Escherichia coli JM83 N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
3.5.2.6 cephalothin + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Escherichia coli JM83 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 penicillin G + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 monomer
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.00833
-
 ceftazidime
-
 Escherichia coli
3.5.2.6 0.183
-
 cefotaxime
-
 Escherichia coli
3.5.2.6 0.4
-
 aztreonam
-
 Escherichia coli
3.5.2.6 0.6
-
 cefepime
-
 Escherichia coli
3.5.2.6 33
-
 cephalosporin C
-
 Escherichia coli
3.5.2.6 41
-
 cephalothin
-
 Escherichia coli
3.5.2.6 770
-
 piperacillin
-
 Escherichia coli
3.5.2.6 780
-
 cephaloridine
-
 Escherichia coli
3.5.2.6 1040
-
 ampicillin
-
 Escherichia coli
3.5.2.6 1570
-
 penicillin G
-
 Escherichia coli