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Literature summary extracted from
- beta-Lactamase (Bacillus cereus) (1975), Methods Enzymol., 43, 640-652.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.2.6 | 1,10-phenanthroline | - |
Bacillus cereus |  |
| 3.5.2.6 | EDTA | - |
Bacillus cereus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.2.6 | 0.06 | - |
benzylpenicillin | - |
Bacillus cereus | |
| 3.5.2.6 | 1.1 | - |
cephalosporin C | beta-lactamse II | Bacillus cereus | |
| 3.5.2.6 | 3.3 | - |
benzylpenicillin | beta-lactamse II | Bacillus cereus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.2.6 | extracellular | - |
Bacillus cereus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.2.6 | Zn2+ | beta-lactamase II | Bacillus cereus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.6 | 22500 | - |
beta-lactamase II, ultracentrifugation | Bacillus cereus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.2.6 | Bacillus cereus | - |
- |
- |
| 3.5.2.6 | Bacillus cereus 5/B | - |
- |
- |
| 3.5.2.6 | Bacillus cereus 569/H/9 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.2.6 | - |
Bacillus cereus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.6 | 5617 | - |
- |
Bacillus cereus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.2.6 | 6-aminopenicillanic acid + H2O | - |
Bacillus cereus | (2R,4S)-2-[amino(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | ampicillin + H2O | - |
Bacillus cereus | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | ampicillin + H2O | - |
Bacillus cereus 569/H/9 | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | ampicillin + H2O | - |
Bacillus cereus 5/B | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | benzylpenicillin + H2O | - |
Bacillus cereus | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | benzylpenicillin + H2O | - |
Bacillus cereus 569/H/9 | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | benzylpenicillin + H2O | - |
Bacillus cereus 5/B | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | cephaloridine + H2O | poor substrate, beta-lactamase I | Bacillus cereus | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
| 3.5.2.6 | cephaloridine + H2O | poor substrate, beta-lactamase I | Bacillus cereus 569/H/9 | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
| 3.5.2.6 | cephaloridine + H2O | poor substrate, beta-lactamase I | Bacillus cereus 5/B | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
| 3.5.2.6 | cephalosporin C + H2O | poor substrate, beta-lactamse I | Bacillus cereus | N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine | - |
? | |
| 3.5.2.6 | cephalosporin C + H2O | poor substrate, beta-lactamse I | Bacillus cereus 569/H/9 | N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine | - |
? | |
| 3.5.2.6 | cephalosporin C + H2O | poor substrate, beta-lactamse I | Bacillus cereus 5/B | N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine | - |
? | |
| 3.5.2.6 | methicillin + H2O | - |
Bacillus cereus | (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | methicillin + H2O | - |
Bacillus cereus 569/H/9 | (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | methicillin + H2O | - |
Bacillus cereus 5/B | (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.2.6 | beta-lactamase I-III | - |
Bacillus cereus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.6 | 50 | - |
denaturation after 10 min | Bacillus cereus |
| 3.5.2.6 | 60 | - |
total loss of activity after 10 min, beta-lactamase I | Bacillus cereus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.6 | 6 | 7 | beta-lactamase II | Bacillus cereus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.6 | 5 | 9 | - |
Bacillus cereus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.6 | 5 | 9 | - |
Bacillus cereus |
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