BRENDA - Enzyme Database

Purification and characterization of the D-hydantoinase from Bacillus circulans

Luksa, V.; Starkuviene, V.; Starkuviene, B.; Dagys, R.; Appl. Biochem. Biotechnol. 62, 219-230 (1997)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.5.2.2
synthesis
commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals
Geobacillus stearothermophilus
3.5.2.2
synthesis
commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals
Bacillus circulans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.2.2
p-hydroxymercuribenzoate
-
Bacillus circulans
3.5.2.2
p-hydroxymercuribenzoate
-
Geobacillus stearothermophilus
3.5.2.2
p-hydroxymercuribenzoate
-
Pseudomonas fluorescens
3.5.2.2
p-hydroxymercuribenzoate
-
Pseudomonas putida
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.2.2
4.9
-
DL-5-isobutylhydantoin
-
Bacillus circulans
3.5.2.2
5.8
-
allantoin
-
Bacillus circulans
3.5.2.2
6.8
-
DL-5-benzylhydantoin
-
Bacillus circulans
3.5.2.2
12.8
-
DL-5-butylhydantoin
-
Bacillus circulans
3.5.2.2
17.2
-
DL-5-isopropylhydantoin
-
Bacillus circulans
3.5.2.2
25.6
-
DL-5-propylhydantoin
-
Bacillus circulans
3.5.2.2
27.8
-
DL-5-methylhydantoin
-
Bacillus circulans
3.5.2.2
40.7
-
DL-5-(2-methylthioethyl)hydantoin
-
Bacillus circulans
3.5.2.2
58.8
-
DL-5-phenyllhydantoin
-
Bacillus circulans
3.5.2.2
105.4
-
hydantoin
-
Bacillus circulans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.2.2
53000
-
4 * 53000, SDS-PAGE
Bacillus circulans
3.5.2.2
54000
-
4 * 54000, gel filtration, denaturing conditions
Bacillus circulans
3.5.2.2
212000
-
gel filtration
Bacillus circulans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.5.2.2
hydantoin + H2O
Brevibacillus brevis
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Geobacillus stearothermophilus
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Pseudomonas fluorescens
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Pseudomonas sp.
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Pseudomonas putida
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Bacillus circulans
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Agrobacterium sp.
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Arthrobacter crystallopoietes
-
N-carbamoyl glycine
-
-
?
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.5.2.2
SDS
-
Bacillus circulans
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.5.2.2
Agrobacterium sp.
-
-
-
3.5.2.2
Agrobacterium sp. IP-I 671
-
-
-
3.5.2.2
Arthrobacter crystallopoietes
-
-
-
3.5.2.2
Bacillus circulans
-
-
-
3.5.2.2
Brevibacillus brevis
-
L-specific hydantoinase
-
3.5.2.2
Geobacillus stearothermophilus
-
-
-
3.5.2.2
Pseudomonas fluorescens
-
-
-
3.5.2.2
Pseudomonas putida
-
-
-
3.5.2.2
Pseudomonas sp.
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.5.2.2
-
Bacillus circulans
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.2.2
43.8
-
-
Bacillus circulans
Storage Stability
EC Number
Storage Stability
Organism
3.5.2.2
-20°C, 5 mM Tris buffer pH 8.5, 0.05 mM MnCl2, 50% initial activity left after 6 months
Bacillus circulans
3.5.2.2
4°C, 5 mM Tris buffer pH 8.5, 0.05 mM MnCl2, retains full activity 1 month
Bacillus circulans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.5.2.2
allantoin + H2O
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
allantoin + H2O
-
209297
Agrobacterium sp.
?
-
-
-
?
3.5.2.2
allantoin + H2O
-
209297
Agrobacterium sp. IP-I 671
?
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Pseudomonas fluorescens
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Pseudomonas putida
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Bacillus circulans
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Agrobacterium sp.
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Arthrobacter crystallopoietes
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Agrobacterium sp. IP-I 671
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Pseudomonas fluorescens
?
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Pseudomonas putida
?
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Agrobacterium sp.
?
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Arthrobacter crystallopoietes
?
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Agrobacterium sp. IP-I 671
?
-
-
-
?
3.5.2.2
DL-5-butylhydantoin
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
DL-5-isobutyl-hydantoin + H2O
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
DL-5-isobutyl-hydantoin + H2O
-
209297
Agrobacterium sp.
?
-
-
-
?
3.5.2.2
DL-5-isobutyl-hydantoin + H2O
-
209297
Agrobacterium sp. IP-I 671
?
-
-
-
?
3.5.2.2
DL-5-isopropylhydantoin + H2O
-
209297
Pseudomonas fluorescens
N-carbamoyl-D-valine
-
-
-
?
3.5.2.2
DL-5-isopropylhydantoin + H2O
-
209297
Bacillus circulans
N-carbamoyl-D-valine
-
-
-
?
3.5.2.2
DL-5-isopropylhydantoin + H2O
-
209297
Agrobacterium sp.
N-carbamoyl-D-valine
-
-
-
?
3.5.2.2
DL-5-isopropylhydantoin + H2O
-
209297
Agrobacterium sp. IP-I 671
N-carbamoyl-D-valine
-
-
-
?
3.5.2.2
DL-5-methylhydantoin
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
DL-5-phenylhydantoin + H2O
-
209297
Geobacillus stearothermophilus
N-carbamoyl-D-phenylglycine
-
-
-
?
3.5.2.2
DL-5-phenylhydantoin + H2O
-
209297
Pseudomonas putida
N-carbamoyl-D-phenylglycine
-
-
-
?
3.5.2.2
DL-5-phenylhydantoin + H2O
-
209297
Bacillus circulans
N-carbamoyl-D-phenylglycine
-
-
-
?
3.5.2.2
DL-5-phenylhydantoin + H2O
-
209297
Arthrobacter crystallopoietes
N-carbamoyl-D-phenylglycine
-
-
-
?
3.5.2.2
DL-5-propylhydantoin
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Brevibacillus brevis
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Geobacillus stearothermophilus
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Pseudomonas fluorescens
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Pseudomonas sp.
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Pseudomonas putida
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Bacillus circulans
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Agrobacterium sp.
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Arthrobacter crystallopoietes
N-carbamoyl glycine
-
-
-
?
3.5.2.2
additional information
-
209297
Pseudomonas putida
?
-
-
-
?
3.5.2.2
additional information
5,5-dimethylhydantoin is no substrate
209297
Bacillus circulans
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.2.2
tetramer
-
Geobacillus stearothermophilus
3.5.2.2
tetramer
-
Pseudomonas fluorescens
3.5.2.2
tetramer
-
Arthrobacter crystallopoietes
3.5.2.2
tetramer
4 * 53000, SDS-PAGE
Bacillus circulans
3.5.2.2
tetramer
4 * 54000, gel filtration, denaturing conditions
Bacillus circulans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.2.2
75
-
DL-5-isopropylhydantoin as substrate
Bacillus circulans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.2.2
60
-
-
Geobacillus stearothermophilus
3.5.2.2
60
-
-
Pseudomonas putida
3.5.2.2
60
-
stable up to, retains full activity on heating at 40°C for 8 h, retains 75% original activity at 62.5°C for 8 h
Bacillus circulans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.2.2
8
10
-
Bacillus circulans
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.2.2
8.5
9.5
most stable in this range
Bacillus circulans
Application (protein specific)
EC Number
Application
Commentary
Organism
3.5.2.2
synthesis
commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals
Geobacillus stearothermophilus
3.5.2.2
synthesis
commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals
Bacillus circulans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.2.2
p-hydroxymercuribenzoate
-
Geobacillus stearothermophilus
3.5.2.2
p-hydroxymercuribenzoate
-
Pseudomonas fluorescens
3.5.2.2
p-hydroxymercuribenzoate
-
Pseudomonas putida
3.5.2.2
p-hydroxymercuribenzoate
-
Bacillus circulans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.2.2
4.9
-
DL-5-isobutylhydantoin
-
Bacillus circulans
3.5.2.2
5.8
-
allantoin
-
Bacillus circulans
3.5.2.2
6.8
-
DL-5-benzylhydantoin
-
Bacillus circulans
3.5.2.2
12.8
-
DL-5-butylhydantoin
-
Bacillus circulans
3.5.2.2
17.2
-
DL-5-isopropylhydantoin
-
Bacillus circulans
3.5.2.2
25.6
-
DL-5-propylhydantoin
-
Bacillus circulans
3.5.2.2
27.8
-
DL-5-methylhydantoin
-
Bacillus circulans
3.5.2.2
40.7
-
DL-5-(2-methylthioethyl)hydantoin
-
Bacillus circulans
3.5.2.2
58.8
-
DL-5-phenyllhydantoin
-
Bacillus circulans
3.5.2.2
105.4
-
hydantoin
-
Bacillus circulans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.2.2
53000
-
4 * 53000, SDS-PAGE
Bacillus circulans
3.5.2.2
54000
-
4 * 54000, gel filtration, denaturing conditions
Bacillus circulans
3.5.2.2
212000
-
gel filtration
Bacillus circulans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.5.2.2
hydantoin + H2O
Brevibacillus brevis
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Geobacillus stearothermophilus
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Pseudomonas fluorescens
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Pseudomonas sp.
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Pseudomonas putida
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Bacillus circulans
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Agrobacterium sp.
-
N-carbamoyl glycine
-
-
?
3.5.2.2
hydantoin + H2O
Arthrobacter crystallopoietes
-
N-carbamoyl glycine
-
-
?
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.5.2.2
SDS
-
Bacillus circulans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.2.2
-
Bacillus circulans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.2.2
43.8
-
-
Bacillus circulans
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.2.2
-20°C, 5 mM Tris buffer pH 8.5, 0.05 mM MnCl2, 50% initial activity left after 6 months
Bacillus circulans
3.5.2.2
4°C, 5 mM Tris buffer pH 8.5, 0.05 mM MnCl2, retains full activity 1 month
Bacillus circulans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.5.2.2
allantoin + H2O
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
allantoin + H2O
-
209297
Agrobacterium sp.
?
-
-
-
?
3.5.2.2
allantoin + H2O
-
209297
Agrobacterium sp. IP-I 671
?
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Pseudomonas fluorescens
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Pseudomonas putida
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Bacillus circulans
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Agrobacterium sp.
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Arthrobacter crystallopoietes
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
209297
Agrobacterium sp. IP-I 671
N-carbamoyl-D-methionine
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Pseudomonas fluorescens
?
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Pseudomonas putida
?
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Agrobacterium sp.
?
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Arthrobacter crystallopoietes
?
-
-
-
?
3.5.2.2
DL-5-benzylhydantoin + H2O
-
209297
Agrobacterium sp. IP-I 671
?
-
-
-
?
3.5.2.2
DL-5-butylhydantoin
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
DL-5-isobutyl-hydantoin + H2O
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
DL-5-isobutyl-hydantoin + H2O
-
209297
Agrobacterium sp.
?
-
-
-
?
3.5.2.2
DL-5-isobutyl-hydantoin + H2O
-
209297
Agrobacterium sp. IP-I 671
?
-
-
-
?
3.5.2.2
DL-5-isopropylhydantoin + H2O
-
209297
Pseudomonas fluorescens
N-carbamoyl-D-valine
-
-
-
?
3.5.2.2
DL-5-isopropylhydantoin + H2O
-
209297
Bacillus circulans
N-carbamoyl-D-valine
-
-
-
?
3.5.2.2
DL-5-isopropylhydantoin + H2O
-
209297
Agrobacterium sp.
N-carbamoyl-D-valine
-
-
-
?
3.5.2.2
DL-5-isopropylhydantoin + H2O
-
209297
Agrobacterium sp. IP-I 671
N-carbamoyl-D-valine
-
-
-
?
3.5.2.2
DL-5-methylhydantoin
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
DL-5-phenylhydantoin + H2O
-
209297
Geobacillus stearothermophilus
N-carbamoyl-D-phenylglycine
-
-
-
?
3.5.2.2
DL-5-phenylhydantoin + H2O
-
209297
Pseudomonas putida
N-carbamoyl-D-phenylglycine
-
-
-
?
3.5.2.2
DL-5-phenylhydantoin + H2O
-
209297
Bacillus circulans
N-carbamoyl-D-phenylglycine
-
-
-
?
3.5.2.2
DL-5-phenylhydantoin + H2O
-
209297
Arthrobacter crystallopoietes
N-carbamoyl-D-phenylglycine
-
-
-
?
3.5.2.2
DL-5-propylhydantoin
-
209297
Bacillus circulans
?
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Brevibacillus brevis
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Geobacillus stearothermophilus
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Pseudomonas fluorescens
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Pseudomonas sp.
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Pseudomonas putida
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Bacillus circulans
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Agrobacterium sp.
N-carbamoyl glycine
-
-
-
?
3.5.2.2
hydantoin + H2O
-
209297
Arthrobacter crystallopoietes
N-carbamoyl glycine
-
-
-
?
3.5.2.2
additional information
-
209297
Pseudomonas putida
?
-
-
-
?
3.5.2.2
additional information
5,5-dimethylhydantoin is no substrate
209297
Bacillus circulans
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.2.2
tetramer
-
Geobacillus stearothermophilus
3.5.2.2
tetramer
-
Pseudomonas fluorescens
3.5.2.2
tetramer
-
Arthrobacter crystallopoietes
3.5.2.2
tetramer
4 * 53000, SDS-PAGE
Bacillus circulans
3.5.2.2
tetramer
4 * 54000, gel filtration, denaturing conditions
Bacillus circulans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.2.2
75
-
DL-5-isopropylhydantoin as substrate
Bacillus circulans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.2.2
60
-
-
Geobacillus stearothermophilus
3.5.2.2
60
-
-
Pseudomonas putida
3.5.2.2
60
-
stable up to, retains full activity on heating at 40°C for 8 h, retains 75% original activity at 62.5°C for 8 h
Bacillus circulans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.2.2
8
10
-
Bacillus circulans
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.2.2
8.5
9.5
most stable in this range
Bacillus circulans