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Literature summary extracted from

  • Ishikawa, T.; Mukohara, Y.; Watabe, K.; Kobayashi, S.; Nakamura, H.
    Microbial conversion of DL-5-substituted hydantoins to the corresponding L-amino acids by Bacillus stearothermophilus NS1122A (1994), Biosci. Biotechnol. Biochem., 58, 265-270.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.2 DL-hydantoinase cloned from 172-kb native plasmid Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 Cu2+ CuSO4*5H2O strongly inhibits L-methionine production Geobacillus stearothermophilus
3.5.2.2 Cu2+
-
 Pseudomonas fluorescens
3.5.2.2 Zn2+ ZnSO4*7H2O strongly inhibits L-methionine production Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.87 Co2+ 0.5 mM, strong activation, no activity of partially purified enzyme without Co2+, Mn2+ or Ni2+ Geobacillus stearothermophilus
3.5.1.87 Mn2+ 0.5 mM, strong activation, no activity of partially purified enzyme without Co2+, Mn2+ or Ni2+ Geobacillus stearothermophilus
3.5.1.87 Ni2+ 5.0 mM, strong activation, no activity of partially purified enzyme without Co2+, Mn2+ or Ni2+ Geobacillus stearothermophilus
3.5.2.2 Co2+
-
 Bos taurus
3.5.2.2 Co2+ stimulates production of L-methionine Geobacillus stearothermophilus
3.5.2.2 Mn2+
-
 Bos taurus
3.5.2.2 Mn2+
-
 Thermus sp.
3.5.2.2 Mn2+ stimulates production of L-methionine Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 55000
-
 4 * 55000, SDS-PAGE Geobacillus stearothermophilus
3.5.2.2 155000 200000
-
 Arthrobacter sp.
3.5.2.2 200000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 hydantoin + H2O Bos taurus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Geobacillus stearothermophilus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas fluorescens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Arthrobacter sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Thermus sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Flavobacterium sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas sp. NS671
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Arthrobacter sp. DSM 3747
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Flavobacterium sp. AJ-3912
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.87 Geobacillus stearothermophilus
-
-
-
3.5.1.87 Geobacillus stearothermophilus NS1122A
-
-
-
3.5.2.2 Arthrobacter sp.
-
 DSM3747
-
3.5.2.2 Arthrobacter sp. DSM 3747
-
 DSM3747
-
3.5.2.2 Bos taurus
-
 bovine
-
3.5.2.2 Flavobacterium sp.
-
 AJ-3912
-
3.5.2.2 Flavobacterium sp. AJ-3912
-
 AJ-3912
-
3.5.2.2 Geobacillus stearothermophilus
-
 strains NS1122A and LU1220
-
3.5.2.2 Pseudomonas fluorescens
-
-
-
3.5.2.2 Pseudomonas putida
-
-
-
3.5.2.2 Pseudomonas sp.
-
-
-
3.5.2.2 Pseudomonas sp. NS671
-
-
-
3.5.2.2 Thermus sp.
-
 thermophilic bacterial strains, CBS 303.80 and CBS363.80, asporogenic gram-negative
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.87 ammonium sulfate, phenyl-Sepharose CL-4B, partially purified Geobacillus stearothermophilus
3.5.2.2
-
 Geobacillus stearothermophilus
3.5.2.2
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.87 N-carbamoyl-DL-alanine + H2O
-
 Geobacillus stearothermophilus L-alanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-phenylalanine + H2O 25% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-phenylalanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-serine + H2O 86% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-serine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-threonine + H2O 94% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-threonine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-valine + H2O
-
 Geobacillus stearothermophilus L-valine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-glycine + H2O 71% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus glycine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-asparagine + H2O 52% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-asparagine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-glutamic acid + H2O 56% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-glutamic acid + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-isoleucine + H2O 55% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-isoleucine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-leucine + H2O 9.1% of activity with N-carbamoyl-L-alanine Geobacillus stearothermophilus L-leucine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Geobacillus stearothermophilus L-methionine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-tyrosine + H2O 3% of activity with N-carbamoyl-DL-alanine Geobacillus stearothermophilus L-tyrosine + CO2 + NH3
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Bos taurus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus stearothermophilus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas fluorescens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Thermus sp. 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Flavobacterium sp. 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Flavobacterium sp. AJ-3912 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus stearothermophilus NS1122A 3-ureidopropionate
-
 ?
3.5.2.2 D-5-(1-methylpropyl)hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-isoleucine
-
 ?
3.5.2.2 D-5-(2-methylthioethyl)hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-methionine
-
 ?
3.5.2.2 D-5-benzylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-phenylalanine
-
 ?
3.5.2.2 D-5-isobutylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-leucine
-
 ?
3.5.2.2 D-5-isobutylhydantoin + H2O
-
 Geobacillus stearothermophilus NS1122A N-carbamoyl-D-leucine
-
 ?
3.5.2.2 D-5-isopropylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-valine
-
 ?
3.5.2.2 D-5-isopropylhydantoin + H2O
-
 Geobacillus stearothermophilus NS1122A N-carbamoyl-D-valine
-
 ?
3.5.2.2 D-5-methylhydantoin + H2O
-
 Bos taurus N-carbamoyl-D-alanine
-
 ?
3.5.2.2 D-5-methylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-alanine
-
 ?
3.5.2.2 D-5-methylhydantoin + H2O
-
 Geobacillus stearothermophilus NS1122A N-carbamoyl-D-alanine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bos taurus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Arthrobacter sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Thermus sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Flavobacterium sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas sp. NS671 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Arthrobacter sp. DSM 3747 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Flavobacterium sp. AJ-3912 N-carbamoyl glycine
-
 ?
3.5.2.2 L-5-(1-methylpropyl)hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-L-isoleucine
-
 ?
3.5.2.2 L-5-(2-methylthioethyl)hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-L-methionine
-
 ?
3.5.2.2 L-5-benzylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-L-phenylalanine
-
 ?
3.5.2.2 L-5-isobutylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-L-leucine
-
 ?
3.5.2.2 L-5-isobutylhydantoin + H2O
-
 Geobacillus stearothermophilus NS1122A N-carbamoyl-L-leucine
-
 ?
3.5.2.2 L-5-methylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-L-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 tetramer
-
 Bos taurus
3.5.2.2 tetramer
-
 Pseudomonas fluorescens
3.5.2.2 tetramer 4 * 55000, SDS-PAGE Geobacillus stearothermophilus
3.5.2.2 tetramer 4 * 55000-62000 Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.87 60 70
-
 Geobacillus stearothermophilus
3.5.2.2 60
-
 L-5-methylhydantoin as substrate Geobacillus stearothermophilus
3.5.2.2 60 70 resting cells Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.87 8
-
-
 Geobacillus stearothermophilus
3.5.2.2 8
-
-
 Pseudomonas fluorescens
3.5.2.2 8
-
 resting cells Geobacillus stearothermophilus
3.5.2.2 9.5
-
-
 Pseudomonas putida
3.5.2.2 9.5
-
 L-5-methylhydantoin as substrate Geobacillus stearothermophilus