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Literature summary extracted from

  • Runser, S.; Chinski, N.; Ohleyer, E.
    D-p-Hydroxyphenylglycine production from DL-5-p-hydroxyphenylglycine production from DL-5-p-hydroxyphenylhydantoin by Agrobacterium sp. (1990), Appl. Microbiol. Biotechnol., 33, 382-388.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.2.2 2-Thiouracil induces biosynthesis of enzyme Agrobacterium sp.

Application

EC Number Application Comment Organism
3.5.2.2 medicine D-amino acids for preparation of beta-lactam antibiotics like semisynthetic penicillins and cephalosporins such as ampicillin, amoxicillin and cefadroxyl, peptide hormones and pesticides Pseudomonas putida
3.5.2.2 medicine D-amino acids for preparation of beta-lactam antibiotics like semisynthetic penicillins and cephalosporins such as ampicillin, amoxicillin and cefadroxyl, peptide hormones and pesticides Agrobacterium sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.2
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas fluorescens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas putida
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Agrobacterium tumefaciens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Arthrobacter crystallopoietes
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Agrobacterium tumefaciens CIP 67.1
-
 3-ureidopropionate
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas fluorescens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium tumefaciens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Arthrobacter crystallopoietes
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Agrobacterium sp.
-
-
-
3.5.2.2 Agrobacterium sp. IP-I 671
-
-
-
3.5.2.2 Agrobacterium tumefaciens
-
-
-
3.5.2.2 Agrobacterium tumefaciens CIP 67.1
-
-
-
3.5.2.2 Arthrobacter crystallopoietes
-
-
-
3.5.2.2 Pseudomonas fluorescens
-
 strain DSM 84
-
3.5.2.2 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Pseudomonas putida
3.5.2.2 partially Agrobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 2-imidazolidone + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 2-thiohydantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 2-thiouracil + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 2-thiouracil + H2O
-
 Agrobacterium sp. IP-I 671 ?
-
 ?
3.5.2.2 5,5-dimethylhydantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O
-
 Arthrobacter crystallopoietes ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O
-
 Agrobacterium sp. IP-I 671 ?
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas fluorescens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium tumefaciens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium sp. 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Arthrobacter crystallopoietes 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium sp. IP-I 671 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium tumefaciens CIP 67.1 3-ureidopropionate
-
 ?
3.5.2.2 6-azauracil + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 6-azauracil + H2O
-
 Agrobacterium sp. IP-I 671 ?
-
 ?
3.5.2.2 allantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 dihydroorotic acid + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Arthrobacter crystallopoietes N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Agrobacterium tumefaciens CIP 67.1 N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Arthrobacter crystallopoietes N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O produces stereospecifically D-p-hydroxyphenylglycine Agrobacterium sp. N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Arthrobacter crystallopoietes ?
-
 ?
3.5.2.2 DL-5-carboxyethylhydantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 DL-5-cyanoethylhydantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 DL-5-isobutyl-hydantoin + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
3.5.2.2 DL-5-isobutyl-hydantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 DL-5-isobutyl-hydantoin + H2O
-
 Agrobacterium tumefaciens CIP 67.1 ?
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Agrobacterium tumefaciens CIP 67.1 N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-sec-butylhydantoin + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
3.5.2.2 DL-5-sec-butylhydantoin + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 DL-5-sec-butylhydantoin + H2O
-
 Agrobacterium tumefaciens CIP 67.1 ?
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Arthrobacter crystallopoietes N-carbamoyl glycine
-
 ?
3.5.2.2 thymine + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 uracil + H2O
-
 Agrobacterium sp. ?
-
 ?
3.5.2.2 uracil + H2O
-
 Agrobacterium sp. IP-I 671 ?
-
 ?
3.5.2.2 uric acid + H2O
-
 Agrobacterium sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.2 45
-
-
 Pseudomonas putida
3.5.2.2 45
-
 resting cells used Agrobacterium sp.
3.5.2.2 50
-
-
 Agrobacterium sp.
3.5.2.2 55
-
-
 Pseudomonas fluorescens
3.5.2.2 55
-
-
 Pseudomonas putida
3.5.2.2 55
-
 purified enzyme Agrobacterium sp.
3.5.2.2 55 60
-
 Agrobacterium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.2 25 75
-
 Agrobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 9
-
-
 Pseudomonas fluorescens
3.5.2.2 9
-
-
 Agrobacterium sp.
3.5.2.2 10
-
-
 Agrobacterium tumefaciens
3.5.2.2 10
-
 purified enzyme Agrobacterium sp.