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Literature summary extracted from

  • Morin, A.; Leblanc, D.; Paleczek, A.; Hummel, W.; Kula, M.R.
    Comparison of seven microbial D-hydantoinases (1990), J. Biotechnol., 16, 37-48.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.2.2 nutrition
-
 Pseudomonas fluorescens
3.5.2.2 nutrition
-
 Pseudomonas putida
3.5.2.2 nutrition
-
 Serratia liquefaciens
3.5.2.2 nutrition
-
 Corynebacterium pseudodiphtheriticum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.2
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 160000
-
 gel filtration Corynebacterium pseudodiphtheriticum
3.5.2.2 190000
-
-
 Pseudomonas putida
3.5.2.2 190000
-
 strain 2.2, gel filtration Pseudomonas putida
3.5.2.2 205000
-
 strain 2.5, gel filtration Pseudomonas putida
3.5.2.2 207000
-
 gel filtration Serratia liquefaciens
3.5.2.2 232000
-
 strain DSM 84, gel filtration Pseudomonas putida
3.5.2.2 235000
-
 strain 1.2 and 1.9, gel filtration Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas fluorescens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas putida
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Serratia liquefaciens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Corynebacterium pseudodiphtheriticum
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Serratia liquefaciens 1.15
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Corynebacterium pseudodiphtheriticum 14.10
-
 3-ureidopropionate
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas fluorescens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Serratia liquefaciens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Corynebacterium pseudodiphtheriticum
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Serratia liquefaciens 1.15
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Corynebacterium pseudodiphtheriticum 14.10
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Corynebacterium pseudodiphtheriticum
-
-
-
3.5.2.2 Corynebacterium pseudodiphtheriticum 14.10
-
-
-
3.5.2.2 Pseudomonas fluorescens
-
 strains 1.2 and 1.9
-
3.5.2.2 Pseudomonas putida
-
-
-
3.5.2.2 Pseudomonas putida
-
 strains 2.2, 2.5
-
3.5.2.2 Pseudomonas putida
-
 DSM 84, formerly classified as P. fluorescens
-
3.5.2.2 Serratia liquefaciens
-
-
-
3.5.2.2 Serratia liquefaciens 1.15
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Pseudomonas putida
3.5.2.2
-
 Serratia liquefaciens
3.5.2.2
-
 Corynebacterium pseudodiphtheriticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas fluorescens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Serratia liquefaciens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Corynebacterium pseudodiphtheriticum 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Serratia liquefaciens 1.15 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Corynebacterium pseudodiphtheriticum 14.10 3-ureidopropionate
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Serratia liquefaciens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Corynebacterium pseudodiphtheriticum N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Serratia liquefaciens 1.15 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Corynebacterium pseudodiphtheriticum 14.10 N-carbamoyl glycine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.2 40 45 strain 2.5 Pseudomonas putida
3.5.2.2 45 55
-
 Serratia liquefaciens
3.5.2.2 45 55
-
 Corynebacterium pseudodiphtheriticum
3.5.2.2 45 55 strain 2.2 and 84 Pseudomonas putida
3.5.2.2 45 55 strain 1.2 Pseudomonas fluorescens
3.5.2.2 57
-
 strain 14.10 Corynebacterium pseudodiphtheriticum
3.5.2.2 57
-
 strain 1.9 Pseudomonas fluorescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.2 22 60
-
 Pseudomonas fluorescens
3.5.2.2 22 60
-
 Pseudomonas putida
3.5.2.2 22 60
-
 Serratia liquefaciens
3.5.2.2 22 60
-
 Corynebacterium pseudodiphtheriticum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.2 30
-
 strain 2.2 and 84, stable activity for at least 50 h Pseudomonas putida
3.5.2.2 37
-
 stable up to at pH 5 Pseudomonas fluorescens
3.5.2.2 37
-
 stable up to at pH 5 Serratia liquefaciens
3.5.2.2 37
-
 strain 2.5 Pseudomonas putida
3.5.2.2 45
-
 strains 1.2 and 1.9 Pseudomonas fluorescens
3.5.2.2 57
-
 stable up to at above pH 8 Corynebacterium pseudodiphtheriticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 8
-
-
 Pseudomonas fluorescens
3.5.2.2 8
-
-
 Serratia liquefaciens
3.5.2.2 8
-
 strain 84 Pseudomonas putida
3.5.2.2 10
-
-
 Corynebacterium pseudodiphtheriticum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.2 6 10
-
 Pseudomonas fluorescens
3.5.2.2 6 10
-
 Pseudomonas putida
3.5.2.2 6 10
-
 Serratia liquefaciens
3.5.2.2 6 10
-
 Corynebacterium pseudodiphtheriticum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.2 5 7 strain 2.2, other strains more stable around pH 5 Pseudomonas putida
3.5.2.2 5
-
-
 Corynebacterium pseudodiphtheriticum
3.5.2.2 5
-
 strain 84 Pseudomonas putida
3.5.2.2 5.5 8.5
-
 Pseudomonas fluorescens
3.5.2.2 5.5 8.5
-
 Pseudomonas putida