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Literature summary extracted from

  • Wallach, D.P.; Grisolia, S.
    The purification and properties of hydropyrimidine hydrase (1956), J. Biol. Chem., 226, 277-288.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 Mg2+ slightly inhibitory with carbamoyl beta-alanine or carbamoyl beta-aminoisobutyric acid as substrates Bos taurus
3.5.2.2 Sn2+
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.2 2.1
-
 Hydrothymine
-
 Bos taurus
3.5.2.2 117.5
-
 hydrouracil
-
 Bos taurus
3.5.2.2 830
-
 hydantoin
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Mg2+ marked acceleration of carbamyl beta-alanine formation from hydrouracil, only slightly stimulatory with hydrothymine as substrate, no effect with hydantoin as substrate Bos taurus
3.5.2.2 Mn2+ marked acceleration of carbamoyl beta-alanine formation from hydrouracil Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 hydantoin + H2O Rattus norvegicus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Bos taurus
-
 N-carbamoyl glycine
-
 ir
3.5.2.2 hydantoin + H2O Columba sp.
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Bos taurus
-
 calf
-
3.5.2.2 Bos taurus
-
 beef
-
3.5.2.2 Columba sp.
-
 pigeon
-
3.5.2.2 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.2 kidney
-
 Rattus norvegicus
-
3.5.2.2 kidney
-
 Bos taurus
-
3.5.2.2 liver
-
 Rattus norvegicus
-
3.5.2.2 liver
-
 Bos taurus
-
3.5.2.2 liver
-
 Columba sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 66.2
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.5.2.2 -20°C, stable for several months Bos taurus
3.5.2.2 4°C, dialyzed against phosphate or veronal buffer, pH 6.9 and pH 8.6, without loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O
-
 Rattus norvegicus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Bos taurus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Columba sp. 3-ureidopropionate
-
 ?
3.5.2.2 carbamoyl beta-aminoisobutyric acid + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.2.2 carbamoyl beta-aminoisobutyric acid + H2O
-
 Bos taurus ?
-
 ?
3.5.2.2 hydantoin + H2O
-
 Rattus norvegicus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bos taurus N-carbamoyl glycine
-
 ir
3.5.2.2 hydantoin + H2O
-
 Columba sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydrothymine
-
 Rattus norvegicus carbamoyl beta-aminoisobutyric acid
-
 ?
3.5.2.2 hydrouracil + H2O
-
 Rattus norvegicus N-carbamoyl beta-alanine
-
 ?
3.5.2.2 additional information
-
 Rattus norvegicus ?
-
 ?
3.5.2.2 additional information inactive with carbamoyl beta-alanine amide, barbituric acid, imidazole 4,5-dicarboxylic acid, 5-(carboxymethylidine)hydantoin, orotic acid, 4,5-aminoimidazolecarboximide, urocanic acid and 5-(diphenyl)hydantoin Bos taurus ?
-
 ?
3.5.2.2 N-carbamoyl beta-alanine + H2O
-
 Rattus norvegicus 5,6-dihydrouracil
-
 r
3.5.2.2 N-carbamoyl beta-alanine + H2O
-
 Bos taurus 5,6-dihydrouracil
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.2 7
-
 Hydrothymine
-
 Bos taurus
3.5.2.2 71.7
-
 hydrouracil
-
 Bos taurus
3.5.2.2 450
-
 hydantoin
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 8.2
-
 borate buffer, hydantoin as substrate Bos taurus
3.5.2.2 8.5
-
 hydantoin as substrate Bos taurus