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Literature summary extracted from

  • Morin, A.; Hummel, W.; Schutte, H.; Kula, M.R.
    Characterization of hydantoinase from Pseudomonas fluorescens strain DSM 84 (1986), Biotechnol. Appl. Biochem., 8, 564-574.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.2.2 nutrition
-
 Pseudomonas putida
3.5.2.2 nutrition useful for production of D-amino acids, microbial hydantoinases used industrially to produce N-carbamoyl-D-hydroxyphenylglycine Pseudomonas fluorescens
3.5.2.2 nutrition useful for production of D-amino acids, microbial hydantoinases used industrially to produce N-carbamoyl-D-hydroxyphenylglycine Pseudomonas putida

General Stability

EC Number General Stability Organism
3.5.2.2 fairly stable during storage in appropriate buffers Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 2,2'-dipyridyl
-
 Pseudomonas fluorescens
3.5.2.2 2,2'-dipyridyl
-
 Pseudomonas putida
3.5.2.2 8-hydroxyquinoline
-
 Pseudomonas fluorescens
3.5.2.2 8-hydroxyquinoline
-
 Pseudomonas putida
3.5.2.2 Cu2+
-
 Pseudomonas fluorescens
3.5.2.2 Cu2+
-
 Pseudomonas putida
3.5.2.2 p-hydroxymercuribenzoate
-
 Pseudomonas fluorescens
3.5.2.2 p-hydroxymercuribenzoate
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.2 11
-
 5,6-dihydrouracil
-
 Pseudomonas fluorescens
3.5.2.2 34
-
 5-isopropylhydantoin
-
 Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Fe2+
-
 Pseudomonas putida
3.5.2.2 Fe2+ needs ferrous ions for activity or reactivation, pure enzyme up to 5 times more active in presence of Fe2+ Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 60000
-
 4 * 60000, SDS-PAGE Pseudomonas fluorescens
3.5.2.2 230000
-
 gel filtration Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas fluorescens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas putida
-
 3-ureidopropionate
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas fluorescens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Pseudomonas fluorescens
-
 strain DSM 84
-
3.5.2.2 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Pseudomonas fluorescens
3.5.2.2
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 0.44
-
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas fluorescens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
3.5.2.2 5-(p-hydroxybenzyl)hydantoin + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.5.2.2 5-(p-hydroxybenzyl)hydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-methionine
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.2 DL-5-benzylhydantoin + H2O poorly hydrolyzed Pseudomonas fluorescens ?
-
 ?
3.5.2.2 DL-5-indolylmethylhydantoin + H2O
-
 Pseudomonas fluorescens L-tryptophan
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl-D-valine
-
 ?
3.5.2.2 hydantoin + H2O poorly hydrolyzed Pseudomonas fluorescens hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 tetramer 4 * 60000, SDS-PAGE Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.2 55
-
-
 Pseudomonas fluorescens
3.5.2.2 55
-
-
 Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.2 30 65
-
 Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.2 40
-
 stable at temperatures up to, starts to lose significant activity at 50°C, remaining activity at 55°C less than 25%, heat treatment in presence of substrate stabilizes enzyme at temperatures in excess of 40°C Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 9
-
 dihydrouracil as substrate Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.2 6 9.5
-
 Pseudomonas fluorescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.2 5.5 8.5
-
 Pseudomonas putida
3.5.2.2 5.5 8.5 most stable in this range, more stable at alkaline pH than at acid pH Pseudomonas fluorescens
3.5.2.2 6 9.5 enzyme inactivated at pH 3.5-4 and above pH 10.5 Pseudomonas fluorescens