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Literature summary extracted from

  • Su, T.M.; Yang, Y.S.
    Identification, purification, and characterization of a thermophilic imidase from pig liver (2000), Protein Expr. Purif., 19, 289-297.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.16 0.35
-
Dihydrothymine pH 9, 60°C Sus scrofa
3.5.2.16 7.7
-
Phthalimide pH 7, 60°C, KM increases about 20-30fold when the reaction temperature increases from 25 to 60°C Sus scrofa
3.5.2.16 10.1
-
hydantoin pH 9, 60°C, KM increases about 20-30fold when the reaction temperature increases from 25 to 60°C Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.16 51000
-
6 * 51000, SDS-PAGE Sus scrofa
3.5.2.16 300000
-
gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.16 dihydrothymine + H2O Sus scrofa
-
?
-
?
3.5.2.16 dihydrouracil + H2O Sus scrofa
-
N-carbamoyl-beta-alanine
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.16 Sus scrofa
-
thermophilic enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.16 salt precipitation, octyl Sepharose, DEAE-Sephacel, chelating Sephacel, gel filtration Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.16 liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.16 13.3
-
measured at 50°C, increasing the reaction temperature from 25 to 60°C leads to an 50 and 60fold increase in specific activity for dihydrouracil and dihydrothymine, respectively, similar activities are found in other mammals Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.16 dihydrothymine + H2O
-
Sus scrofa ?
-
?
3.5.2.16 dihydrouracil + H2O
-
Sus scrofa N-carbamoyl-beta-alanine
-
?
3.5.2.16 hydantoin + H2O
-
Sus scrofa hydantoic acid monoamide
-
?
3.5.2.16 phtalimide + H2O
-
Sus scrofa 2-(aminocarbonyl)benzoic acid
-
?

Subunits

EC Number Subunits Comment Organism
3.5.2.16 hexamer 6 * 51000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.16 60
-
-
Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.16 25 60 imidase acitivity increases with the elevation of temperature except for a reaction at pH 6 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.16 25 60 at a pH around 7.5 and 8.5, imidase is stable at 50°C or at lower temperature for over 2 h, no activity above 70°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.16 7.5 8.5 shifts to more acidic pH at higher temperatures Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.16 6 9 below 40°C, less stable at 50 and 60°C, stability decreases in acidic or alkaline pH at 60°C Sus scrofa