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Literature summary extracted from

  • Hayaishi, O.; Kornberg, A.
    Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes (1952), J. Biol. Chem., 197, 717-732.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.99.4 methylene blue required as electron acceptor Corynebacterium sp.
1.17.99.4 methylene blue required as electron acceptor Mycobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.99.4 0.035
-
 thymine
-
 Mycobacterium sp.
1.17.99.4 0.131
-
 Uracil
-
 Mycobacterium sp.
3.5.2.1 3.37
-
 Barbiturate
-
 Mycobacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.99.4 cytoplasm
-
 Corynebacterium sp. 5737
-
1.17.99.4 cytoplasm
-
 Mycobacterium sp. 5737
-

Organism

EC Number Organism UniProt Comment Textmining
1.17.99.4 Corynebacterium sp.
-
-
-
1.17.99.4 Corynebacterium sp. 161
-
-
-
1.17.99.4 Mycobacterium sp.
-
-
-
3.5.2.1 Mycobacterium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.1
-
 Mycobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.1 additional information
-
-
 Mycobacterium sp.

Storage Stability

EC Number Storage Stability Organism
1.17.99.4 -10°C, most stable at pH 9.0, no appreciable loss of activity for several months Mycobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.99.4 thymine + acceptor
-
 Corynebacterium sp. 5-methylbarbiturate + reduced acceptor
-
 ?
1.17.99.4 thymine + acceptor
-
 Mycobacterium sp. 5-methylbarbiturate + reduced acceptor
-
 ?
1.17.99.4 thymine + acceptor
-
 Corynebacterium sp. 161 5-methylbarbiturate + reduced acceptor
-
 ?
1.17.99.4 uracil + acceptor barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates Corynebacterium sp. barbiturate + reduced acceptor
-
 ?
1.17.99.4 uracil + acceptor barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates Mycobacterium sp. barbiturate + reduced acceptor
-
 ?
1.17.99.4 uracil + acceptor barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates Corynebacterium sp. 161 barbiturate + reduced acceptor
-
 ?
3.5.2.1 barbiturate + 2 H2O
-
 Mycobacterium sp. malonate + urea
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.99.4 22 25 assay at Corynebacterium sp.
1.17.99.4 22 25 assay at Mycobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.99.4 8.5
-
-
 Mycobacterium sp.
3.5.2.1 8 9
-
 Mycobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.99.4 8 10 about half-maximal activity at pH 8.0 and 10 with thymine as substrate Mycobacterium sp.
1.17.99.4 8 9.7 about half-maximal activity at pH 8.0 and 9.7 with uracil as substrate Mycobacterium sp.
3.5.2.1 7.5 9.5 pH 7.5: about 70% of maximal activity, pH 9.5: about 45% of maximal activity Mycobacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.17.99.4 7
-
 90% loss of activity Mycobacterium sp.
1.17.99.4 9
-
 most stable at -10°C, no appreciable loss of activity for several months Mycobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.99.4 additional information no oxidation in the presence of O2 without methylene blue as electron acceptor Corynebacterium sp.
1.17.99.4 additional information no oxidation in the presence of O2 without methylene blue as electron acceptor Mycobacterium sp.