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Literature summary extracted from

  • Jakoby, W.B.
    Kynurenine fromamidase from Neurospora (1954), J. Biol. Chem., 207, 657-663.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.9 N-acetylanthranilic acid 15% inhibition at 6.7 mM Neurospora crassa
3.5.1.9 N-formyl-o-aminotoluene 45% inhibition at 6.7 mM Neurospora crassa
3.5.1.9 Na-bisulfite complete inhibition at 4 mM Neurospora crassa
3.5.1.9 NaCN 31% inhibition at 5 mM Neurospora crassa
3.5.1.9 NaHSO3 complete inhibition at 4 mM Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.9 0.11
-
 N-formyl-L-kynurenine
-
 Neurospora crassa
3.5.1.9 2.5
-
 N-formyl-o-aminonitrobenzene
-
 Neurospora crassa
3.5.1.9 2.8
-
 N-formyl-o-aminoacetophenone
-
 Neurospora crassa
3.5.1.9 3.3
-
 N-formylanthranilic acid
-
 Neurospora crassa
3.5.1.9 7.7
-
 N-formyl-m-aminobenzoic acid
-
 Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.9 N-formyl-L-kynurenine + H2O Neurospora crassa
-
 L-kynurenine + formate
-
 ?
3.5.1.9 N-formyl-m-aminobenzoic acid + H2O Neurospora crassa very low activity formate + m-aminobenzoic acid
-
 ?
3.5.1.9 N-formyl-o-aminoacetophenone + H2O Neurospora crassa 22% of activity compared to N-formyl-L-kynurenine formate + o-aminoacetophenone
-
 ?
3.5.1.9 N-formyl-o-aminonitrobenzene + H2O Neurospora crassa low activity formate + o-nitroaniline
-
 ?
3.5.1.9 N-formylanthranilic acid + H2O Neurospora crassa 24% of activity compared to N-formyl-L-kynurenine formate + anthranilic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.9 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.9 partial Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.9 cell culture
-
 Neurospora crassa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.9 N-formyl-L-kynurenine + H2O
-
 Neurospora crassa L-kynurenine + formate
-
 ?
3.5.1.9 N-formyl-m-aminobenzoic acid + H2O very low activity Neurospora crassa formate + m-aminobenzoic acid
-
 ?
3.5.1.9 N-formyl-o-aminoacetophenone + H2O 22% of activity compared to N-formyl-L-kynurenine Neurospora crassa formate + o-aminoacetophenone
-
 ?
3.5.1.9 N-formyl-o-aminonitrobenzene + H2O low activity Neurospora crassa formate + o-nitroaniline
-
 ?
3.5.1.9 N-formylanthranilic acid + H2O 24% of activity compared to N-formyl-L-kynurenine Neurospora crassa formate + anthranilic acid
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.9 56
-
 30% inactivation after 2 min Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.9 7.3 7.8
-
 Neurospora crassa