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Literature summary extracted from

  • Kobashi, K.; Takebe, S.; Sakai, T.
    Urethane-hydrolyzing enzyme from Citrobacter sp (1990), Chem. Pharm. Bull., 38, 1326-1328.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.75 nutrition practically ineffective for the elimination of urethane from alcoholic beverages, because the enzyme is inactive in high concentrations of alcohol, ethanol, and at acidic pH Citrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.75 Ag+ 1 mM: complete inhibition Citrobacter sp.
3.5.1.75 EDTA no inhibition Citrobacter sp.
3.5.1.75 F- 1 mM: complete inhibition Citrobacter sp.
3.5.1.75 Hg2+ 1 mM: complete inhibition Citrobacter sp.
3.5.1.75 iodoacetamide no inhibition at 0.1 mM Citrobacter sp.
3.5.1.75 additional information no inhibition by 1 mM EDTA and 2-mercaptoethanol; no inhibition by diethyldicarbonate, N-ethylmaleimide or iodoacetamide at 0.1 mM Citrobacter sp.
3.5.1.75 N-Benzoylphosphoric triamide
-
 Citrobacter sp.
3.5.1.75 O,O-Dimethyl-O-(2,2-dichlorovinyl) phosphate
-
 Citrobacter sp.
3.5.1.75 O,O-Dimethyl-O-(3-methyl-4-nitrophenyl) phosphate
-
 Citrobacter sp.
3.5.1.75 phenylphosphorodiamide
-
 Citrobacter sp.
3.5.1.75 phenylthiophosphorodiamide
-
 Citrobacter sp.
3.5.1.75 Sodium laurylsulfate 35 mM: complete inhibition Citrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.75 1.6
-
 urethane
-
 Citrobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.75 cytosol 80% of activity in a cytosolic fraction Citrobacter sp. 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.75 additional information metal ions not required Citrobacter sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.5.1.75 Ethanol inactive at high concentrations of alcohol, 20% ethanol, 8.9% residual activity after 2 h at 37°C Citrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.75 Citrobacter sp.
-
 isolated from mouse feces
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.75 partial Citrobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.75 cell culture urethanase activity detected in the cells not in the culture medium Citrobacter sp.
-
3.5.1.75 additional information urethanase activity detected in the cells not in the culture medium Citrobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.75 additional information
-
-
 Citrobacter sp.
3.5.1.75 0.254
-
-
 Citrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.75 acetamide + H2O 880% of the activity with ethylcarbamate Citrobacter sp. ?
-
 ?
3.5.1.75 benzamide + H2O 680% of the activity with ethylcarbamate Citrobacter sp. ?
-
 ?
3.5.1.75 benzylcarbamate + H2O 148% of the activity with ethylcarbamate Citrobacter sp. CO2 + NH3 + benzoate
-
 ?
3.5.1.75 ethylcarbamate + H2O urethane, potentially carcinogenic, mutagenic and teratogenic to human Citrobacter sp. CO2 + NH3 + ethanol equimolar amounts of ammonia and ethanol ?
3.5.1.75 ethylcarbamate + H2O urethane, potentially carcinogenic, mutagenic and teratogenic to human Citrobacter sp. CO2 + NH3 + ethanol noncarcinogenic compounds ?
3.5.1.75 methylcarbamate + H2O 64% of the activity with ethylcarbamate Citrobacter sp. CO2 + NH3 + methanol
-
 ?
3.5.1.75 additional information substrate specificity Citrobacter sp. ?
-
 ?
3.5.1.75 additional information no activity with urea, methylurea, ethylurea, n-butylurea, tert-butylurea, phenylurea Citrobacter sp. ?
-
 ?
3.5.1.75 additional information no activity with glycinamide, N-alkyl ureas, N-allylurea, ethyl esters of organic acids, ethyl acetate, ethyl benzoate, diethyl carbonate, phenylphosphorodiamide, phenylthiophosphorodiamide, N-benzoylphosphoric triamide Citrobacter sp. ?
-
 ?
3.5.1.75 n-butylcarbamate + H2O 224% of the activity with ethylcarbamate Citrobacter sp. CO2 + NH3 + n-butanol
-
 ?
3.5.1.75 n-butyramide + H2O 1000% of the activity with ethylcarbamate Citrobacter sp. ?
-
 ?
3.5.1.75 phenylcarbamate + H2O 96% of the activity with ethylcarbamate Citrobacter sp. CO2 + NH3 + phenol
-
 ?
3.5.1.75 tert-butylcarbamate + H2O 24.4% of the activity with ethylcarbamate Citrobacter sp. CO2 + NH3 + tert-butanol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.75 37
-
 assay at Citrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.75 65
-
 15 min, complete loss of activity Citrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.75 6 8
-
 Citrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.75 5
-
 half of maximum activity at pH 5.0 Citrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
3.5.1.75 additional information does not require any cofactor Citrobacter sp.