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Literature summary extracted from

  • Inokoshi, J.; Takeshima, H.; Ikeda, H.; Omura, S.
    Cloning and sequencing of the aculeacin A acylase-encoding gene from Actinoplanes utahensis and expression in Streptomyces lividans (1992), Gene, 119, 29-35.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.70 pharmacology useful in producing peptide nuclei, i.e. deacetylated cyclic hexapeptides, for creating new antifungal agents by introducing different acyl moieties Actinoplanes utahensis
3.5.1.70 synthesis useful in producing peptide nuclei for creating new antifungal agents by introducing different acyl moieties Actinoplanes utahensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.70 expression in Streptomyces lividans JT46 Actinoplanes utahensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.70 extracellular
-
 Actinoplanes utahensis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.70 19100
-
 1 * 60300 + 1 * 19100, two dissimilar subunits, calculated from nucleotide sequence Actinoplanes utahensis
3.5.1.70 60300
-
 1 * 60300 + 1 * 19100, two dissimilar subunits, calculated from nucleotide sequence Actinoplanes utahensis
3.5.1.70 84070
-
 precursor peptide of aculeacin A acylase, calculated from nucleotide sequence Actinoplanes utahensis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.70 Actinoplanes utahensis
-
 NRRL 12052
-
3.5.1.70 Actinoplanes utahensis NRRL 12052
-
 NRRL 12052
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.70 proteolytic modification enzyme is translated as single precursor polypeptide and then processed by proteolytic modification to the active form consisting of two subunits Actinoplanes utahensis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.70 from recombinant strain Streptomyces lividans JT46 Actinoplanes utahensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.70 culture filtrate from recombinant strain Streptomyces lividans, about 80% detected in culture filtrate Actinoplanes utahensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.70 11.1
-
 from recombinant strain Streptomyces lividans JT46 Actinoplanes utahensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.70 aculeacin A + H2O
-
 Actinoplanes utahensis cyclo-hexapeptide + palmitic acid
-
 ?
3.5.1.70 aculeacin A + H2O
-
 Actinoplanes utahensis NRRL 12052 cyclo-hexapeptide + palmitic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.70 dimer 1 * 60300 + 1 * 19100, two dissimilar subunits, calculated from nucleotide sequence Actinoplanes utahensis
3.5.1.70 More enzyme is translated as single precursor polypeptide and then processed by proteolytic processing to the active form consisting of two subunits Actinoplanes utahensis

Synonyms

EC Number Synonyms Comment Organism
3.5.1.70 AAC
-
 Actinoplanes utahensis