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Literature summary extracted from

  • Inokoshi, J.; Takeshima, H.; Omura, S.
    Identification of precursor peptide of aculeacin A acylase as a protein with proteolytic activity (1993), FEMS Microbiol. Lett., 114, 305-310.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.70 extracellular
-
 Actinoplanes utahensis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.70 87000
-
 precursor peptide of aculeacin A acylase, gel filtration and SDS-PAGE Actinoplanes utahensis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.70 Actinoplanes utahensis
-
 NRRL 12052
-
3.5.1.70 Actinoplanes utahensis NRRL 12052
-
 NRRL 12052
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.70 proteolytic modification the 87000 Da enzyme precursor protein is processed post-translationally at 37°C, 0.1% SDS for 1 h to 60000 Da and 19000 Da peptides by its own proteolytic activity Actinoplanes utahensis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.70 enzyme precursor peptide Actinoplanes utahensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.70 culture filtrate
-
 Actinoplanes utahensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.70 additional information
-
-
 Actinoplanes utahensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.70 aculeacin A + H2O
-
 Actinoplanes utahensis cyclo-hexapeptide + palmitic acid
-
 ?
3.5.1.70 aculeacin A + H2O
-
 Actinoplanes utahensis NRRL 12052 cyclo-hexapeptide + palmitic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.70 dimer
-
 Actinoplanes utahensis
3.5.1.70 monomer 1 * 87000, SDS-PAGE and gel filtration, precursor peptide of aculeacin A acylase Actinoplanes utahensis
3.5.1.70 More the 87000 Da enzyme precursor protein is processed post-translationally at 37°C, 0.1% SDS for 1 h to 60000 Da and 19000 Da peptides by its own proteolytic activity Actinoplanes utahensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.70 60
-
-
 Actinoplanes utahensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.70 7
-
-
 Actinoplanes utahensis