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Literature summary extracted from

  • Soda, K.; Uchiyama, K.; Ogata, K.
    Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part I. Bacterial and enzymatic hydrolysis of L- and D-isomers of theanine and identification of products (1966), Agric. Biol. Chem., 30, 541-546.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.65 D-glutamate activation and induction Pseudomonas aeruginosa
3.5.1.65 L-glutamate activation and induction Pseudomonas aeruginosa
3.5.1.65 L-Theanine activation and induction Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.65 N5-ethyl-L-glutamine + H2O Pseudomonas aeruginosa N-ethyl-gamma-L-glutamine L-glutamate + ethylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.65 Pseudomonas aeruginosa
-
-
-
3.5.1.65 Pseudomonas fluorescens
-
-
-
3.5.1.65 Pseudomonas sp.
-
 T-1
-
3.5.1.65 Pseudomonas sp. T-1
-
 T-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.65 partial, glutaminase could not be separated Pseudomonas aeruginosa

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.65 N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine stoichiometry of reaction Pseudomonas fluorescens
a
3.5.1.65 N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine stoichiometry of reaction Pseudomonas aeruginosa
a
3.5.1.65 N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine stoichiometry of reaction Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.65 additional information
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.65 N5-ethyl-L-glutamine + H2O
-
 Pseudomonas fluorescens L-glutamate + ethylamine
-
 ?
3.5.1.65 N5-ethyl-L-glutamine + H2O
-
 Pseudomonas aeruginosa L-glutamate + ethylamine
-
 ?
3.5.1.65 N5-ethyl-L-glutamine + H2O
-
 Pseudomonas sp. L-glutamate + ethylamine
-
 ?
3.5.1.65 N5-ethyl-L-glutamine + H2O N-ethyl-gamma-L-glutamine Pseudomonas aeruginosa L-glutamate + ethylamine
-
 ?
3.5.1.65 N5-ethyl-L-glutamine + H2O
-
 Pseudomonas sp. T-1 L-glutamate + ethylamine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.65 37
-
 assay at Pseudomonas fluorescens
3.5.1.65 37
-
 assay at Pseudomonas aeruginosa
3.5.1.65 37
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.65 8
-
 assay at Pseudomonas fluorescens
3.5.1.65 8
-
 assay at Pseudomonas aeruginosa
3.5.1.65 8
-
 assay at Pseudomonas sp.