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Literature summary extracted from

  • Shimizu, S.; Kim, J.M.; Shinmen, Y.; Yamada, H.
    Evaluation of two alternative metabolic pathways for creatinine degradation in microorganisms (1986), Arch. Microbiol., 145, 322-328.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.59 N-carbamoylsarcosine + H2O Pseudomonas sp. inducible enzyme sarcosine + CO2 + NH3
-
 ?
3.5.1.59 N-carbamoylsarcosine + H2O Pseudomonas putida inducible enzyme sarcosine + CO2 + NH3
-
 ?
3.5.1.59 N-carbamoylsarcosine + H2O Pseudomonas sp. H21 AKU876 inducible enzyme sarcosine + CO2 + NH3
-
 ?
3.5.4.21 creatinine + H2O Pseudomonas putida
-
 N-methylhydantoin + NH3
-
 ?
3.5.4.21 creatinine + H2O Pseudomonas putida 77
-
 N-methylhydantoin + NH3
-
 ?
3.5.4.21 creatinine + H2O Pseudomonas sp. H21
-
 N-methylhydantoin + NH3
-
 ?
3.5.4.21 creatinine + H2O Pseudomonas sp. 0114
-
 N-methylhydantoin + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.59 Pseudomonas putida
-
-
-
3.5.1.59 Pseudomonas sp.
-
 strains H21 and 0114
-
3.5.3.3 Pseudomonas sp.
-
 H21 AKU876
-
3.5.3.3 Pseudomonas sp. H21 AKU876
-
 H21 AKU876
-
3.5.4.21 Pseudomonas putida
-
-
-
3.5.4.21 Pseudomonas putida 77
-
-
-
3.5.4.21 Pseudomonas sp. 0114
-
-
-
3.5.4.21 Pseudomonas sp. H21
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.21 0.00001
-
 NH4Cl added to culture medium Pseudomonas putida
3.5.4.21 0.0001
-
 NH4Cl added to culture medium Pseudomonas sp. H21
3.5.4.21 0.0001
-
 NH4Cl added to culture medium Pseudomonas sp. 0114
3.5.4.21 0.0002
-
 hydantoin added to culture medium Pseudomonas sp. H21
3.5.4.21 0.00043
-
 creatinine added to culture medium Pseudomonas sp. 0114
3.5.4.21 0.0006
-
 hydantoin added to culture medium Pseudomonas putida
3.5.4.21 0.0011
-
 creatinine added to culture medium Pseudomonas putida
3.5.4.21 0.0012
-
 hydantoin added to culture medium Pseudomonas sp. 0114
3.5.4.21 0.0013
-
-
 Pseudomonas sp. 0114
3.5.4.21 0.0013
-
 N-methylhydantoin added to culture medium Pseudomonas sp. H21
3.5.4.21 0.0014
-
 N-methylhydantoin added to culture medium Pseudomonas putida
3.5.4.21 0.0028
-
 NH4Cl added to culture medium Pseudomonas putida 77
3.5.4.21 0.0058
-
 N-methylhydantoin added to culture medium Pseudomonas sp. H21
3.5.4.21 0.0067
-
 creatinine added to culture medium Pseudomonas sp. H21
3.5.4.21 0.0198
-
 N-methylhydantoin added to culture medium Pseudomonas putida 77
3.5.4.21 0.0452
-
 creatinine added to culture medium Pseudomonas putida 77

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.59 N-carbamoylsarcosine + H2O
-
 Pseudomonas sp. sarcosine + CO2 + NH3
-
 ?
3.5.1.59 N-carbamoylsarcosine + H2O
-
 Pseudomonas putida sarcosine + CO2 + NH3
-
 ?
3.5.1.59 N-carbamoylsarcosine + H2O inducible enzyme Pseudomonas sp. sarcosine + CO2 + NH3
-
 ?
3.5.1.59 N-carbamoylsarcosine + H2O inducible enzyme Pseudomonas putida sarcosine + CO2 + NH3
-
 ?
3.5.1.59 N-carbamoylsarcosine + H2O
-
 Pseudomonas sp. H21 AKU876 sarcosine + CO2 + NH3
-
 ?
3.5.1.59 N-carbamoylsarcosine + H2O inducible enzyme Pseudomonas sp. H21 AKU876 sarcosine + CO2 + NH3
-
 ?
3.5.3.3 creatine + H2O
-
 Pseudomonas sp. sarcosine + urea
-
 ?
3.5.3.3 creatine + H2O
-
 Pseudomonas sp. H21 AKU876 sarcosine + urea
-
 ?
3.5.4.21 creatinine + H2O
-
 Pseudomonas putida N-methylhydantoin + NH3
-
 ?
3.5.4.21 creatinine + H2O
-
 Pseudomonas putida 77 N-methylhydantoin + NH3
-
 ?
3.5.4.21 creatinine + H2O
-
 Pseudomonas sp. H21 N-methylhydantoin + NH3
-
 ?
3.5.4.21 creatinine + H2O
-
 Pseudomonas sp. 0114 N-methylhydantoin + NH3
-
 ?