Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Matsumura, E.; Shin, T.; Murao, S.; Kawano, T.
    Substrate specificity and stoichiometry of Nalpha-benzyloxycarbonyl amino acid urethane hydrolase, from Streptococcus faecalis R ATCC 8043 (1985), Agric. Biol. Chem., 49, 973-979.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.1.58 synthesis can be used in peptide synthesis procedures for removal of the protecting groups Z and Bz from Gly and Ala Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.58 0.1
-
 N-benzyl-Gly
-
 Enterococcus faecalis
3.5.1.58 0.35
-
 N-benzyl-Ala
-
 Enterococcus faecalis
3.5.1.58 0.36
-
 N-Benzyloxycarbonyl-Gly
-
 Enterococcus faecalis
3.5.1.58 0.8
-
 N-Benzyloxycarbonyl-Ala
-
 Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.58 Enterococcus faecalis
-
-
-
3.5.1.58 Enterococcus faecalis R / ATCC 8043
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.58 additional information
-
 specific activity for 14 more substrates Enterococcus faecalis
3.5.1.58 0.0003
-
 for N-benzyloxycarbonyl-Ala Enterococcus faecalis
3.5.1.58 0.0007
-
 for N-benzyl-Ala Enterococcus faecalis
3.5.1.58 0.0067
-
 for N-benzyl-Gly Enterococcus faecalis
3.5.1.58 0.0074
-
 for N-benzyloxylcarbonyl-Gly Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.58 additional information systematic examination on many more substituted amino acids Enterococcus faecalis ?
-
 ?
3.5.1.58 additional information systematic examination on many more substituted amino acids Enterococcus faecalis R / ATCC 8043 ?
-
 ?
3.5.1.58 N-(2-hydroxy)benzyl-Gly + H2O
-
 Enterococcus faecalis 2-hydroxybenzyl alcohol + Gly
-
 ?
3.5.1.58 N-(3-hydroxy)benzyl-Gly + H2O
-
 Enterococcus faecalis 3-hydroxybenzyl alcohol + Gly
-
 ?
3.5.1.58 N-(4-amino)benzyl-Gly + H2O
-
 Enterococcus faecalis 4-aminobenzyl alcohol + Gly
-
 ?
3.5.1.58 N-(4-hydroxy)benzyl-Gly + H2O
-
 Enterococcus faecalis 4-hydroxybenzyl alcohol + Gly
-
 ?
3.5.1.58 N-(4-methoxy)benzyloxycarbonyl-Gly + H2O
-
 Enterococcus faecalis 4-methoxybenzyl alcohol + CO2 + Gly
-
 ?
3.5.1.58 N-benzyl-Gly + H2O
-
 Enterococcus faecalis benzyl alcohol + Gly
-
 ?
3.5.1.58 N-benzyl-Gly + H2O
-
 Enterococcus faecalis R / ATCC 8043 benzyl alcohol + Gly
-
 ?
3.5.1.58 N-benzyl-Gly-Gly + H2O very poor substrate Enterococcus faecalis benzyl alcohol + Gly-Gly
-
 ?
3.5.1.58 N-benzyl-L-Ala + H2O
-
 Enterococcus faecalis benzyl alcohol + Ala
-
 ?
3.5.1.58 N-benzyloxycarbonyl-Gly + H2O
-
 Enterococcus faecalis benzyl alcohol + CO2 + Gly
-
 ?
3.5.1.58 N-benzyloxycarbonyl-Gly + H2O
-
 Enterococcus faecalis R / ATCC 8043 benzyl alcohol + CO2 + Gly
-
 ?
3.5.1.58 N-benzyloxycarbonyl-Gly-Gly + H2O very poor substrate Enterococcus faecalis benzyl alcohol + CO2 + Gly-Gly
-
 ?
3.5.1.58 N-benzyloxycarbonyl-Gly-Gly + H2O very poor substrate Enterococcus faecalis R / ATCC 8043 benzyl alcohol + CO2 + Gly-Gly
-
 ?
3.5.1.58 N-benzyloxycarbonyl-L-Ala + H2O
-
 Enterococcus faecalis benzyl alcohol + CO2 + Ala
-
 ?
3.5.1.58 N-benzyloxycarbonyl-L-Ala + H2O
-
 Enterococcus faecalis R / ATCC 8043 benzyl alcohol + CO2 + Ala
-
 ?