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Literature summary extracted from

  • Murao, S.; Matsumura, E.; Kawano, T.
    Isolation and characterization of a novel enzyme Nalpha-benzyloxycarbonyl amino acid urethane hydrolase, from Streptococcus faecalis R ATCC 8043 (1985), Agric. Biol. Chem., 49, 967-972.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.58 DTT
-
 Enterococcus faecalis
3.5.1.58 EDTA
-
 Enterococcus faecalis
3.5.1.58 iodoacetamide weak Enterococcus faecalis
3.5.1.58 iodoacetate weak Enterococcus faecalis
3.5.1.58 N-benzyloxycarbonyl-L-Phe chloromethyl ketone weak Enterococcus faecalis
3.5.1.58 o-phenanthroline weak Enterococcus faecalis
3.5.1.58 p-chloromercuribenzoate complete loss of activity, inhibition reversible by 2-mercaptoethanol Enterococcus faecalis
3.5.1.58 p-tosyl-L-Lys chloromethyl ketone weak Enterococcus faecalis
3.5.1.58 p-tosyl-L-Phe chloromethyl ketone weak Enterococcus faecalis
3.5.1.58 PMSF weak Enterococcus faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.58 Ba2+ very slight activation Enterococcus faecalis
3.5.1.58 Ca2+ very slight activation Enterococcus faecalis
3.5.1.58 Cd2+ very slight activation Enterococcus faecalis
3.5.1.58 Co2+
-
 Enterococcus faecalis
3.5.1.58 Fe2+ very slight activation Enterococcus faecalis
3.5.1.58 Mn2+
-
 Enterococcus faecalis
3.5.1.58 Pb2+ very slight activation Enterococcus faecalis
3.5.1.58 Zn2+
-
 Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.58 220000
-
 gel filtration Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.58 Enterococcus faecalis
-
-
-
3.5.1.58 Enterococcus faecalis R / ATCC 8043
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.58
-
 Enterococcus faecalis

Subunits

EC Number Subunits Comment Organism
3.5.1.58 octamer octamer postulated, dimer or tetramer possible in case of aggregation of the native enzyme in gel filtration, SDS-PAGE Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.58 35
-
-
 Enterococcus faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.58
-
-
 stable for 60 days at pH 6.0, 80% of activity remaining after 3 months, 50% of activity remaining after 6 months Enterococcus faecalis
3.5.1.58 20
-
 stable for 60 min below 20°C in 0.05 M phosphate buffer, pH 6.0 Enterococcus faecalis
3.5.1.58 25
-
 stable for 16 h around pH 6.0 Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.58 6
-
-
 Enterococcus faecalis