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Literature summary extracted from

  • Murao, S.; Matsumura, E.; Shin, T.; Kawano, T.
    A new enzyme Nalpha-benzyloxy-carbonyl moiety hydrolytic enzyme, from Streptococcus faecalis R (1984), Agric. Biol. Chem., 48, 1673-1675.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.1.58 synthesis can be used in peptide synthesis procedures for removal of the protecting groups Z and Bz from Gly and Ala Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.58 p-chloromercuribenzoate complete loss of activity at concentration of 1 mM Enterococcus faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.58 Co2+ divalent cations required for activity, optimum concentration 1 mM Enterococcus faecalis
3.5.1.58 Zn2+ divalent cations required for activity Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.58 220000
-
 gel filtration Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.58 Enterococcus faecalis
-
-
-
3.5.1.58 Enterococcus faecalis R / ATCC 8043
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.58
-
 Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.58 N-(2-hydroxy)benzyl-Gly + H2O
-
 Enterococcus faecalis 2-hydroxybenzyl alcohol + Gly
-
 ?
3.5.1.58 N-(3-hydroxy)benzyl-Gly + H2O
-
 Enterococcus faecalis 3-hydroxybenzyl alcohol + Gly
-
 ?
3.5.1.58 N-(4-amino)benzyl-Gly + H2O
-
 Enterococcus faecalis 4-aminobenzyl alcohol + Gly
-
 ?
3.5.1.58 N-(4-hydroxy)benzyl-Gly + H2O
-
 Enterococcus faecalis 4-hydroxybenzyl alcohol + Gly
-
 ?
3.5.1.58 N-(4-methoxy)benzyloxycarbonyl-Gly + H2O
-
 Enterococcus faecalis 4-methoxybenzyl alcohol + CO2 + Gly
-
 ?
3.5.1.58 N-benzyl-Gly + H2O
-
 Enterococcus faecalis benzyl alcohol + Gly
-
 ?
3.5.1.58 N-benzyl-Gly + H2O
-
 Enterococcus faecalis R / ATCC 8043 benzyl alcohol + Gly
-
 ?
3.5.1.58 N-benzyl-Gly-Gly + H2O very poor substrate Enterococcus faecalis benzyl alcohol + Gly-Gly
-
 ?
3.5.1.58 N-benzyl-Gly-Gly + H2O very poor substrate Enterococcus faecalis R / ATCC 8043 benzyl alcohol + Gly-Gly
-
 ?
3.5.1.58 N-benzyl-L-Ala + H2O
-
 Enterococcus faecalis benzyl alcohol + Ala
-
 ?
3.5.1.58 N-benzyloxycarbonyl-Gly + H2O
-
 Enterococcus faecalis benzyl alcohol + CO2 + Gly
-
 ?
3.5.1.58 N-benzyloxycarbonyl-Gly + H2O
-
 Enterococcus faecalis R / ATCC 8043 benzyl alcohol + CO2 + Gly
-
 ?
3.5.1.58 N-benzyloxycarbonyl-Gly-Gly + H2O very poor substrate Enterococcus faecalis benzyl alcohol + CO2 + Gly-Gly
-
 ?
3.5.1.58 N-benzyloxycarbonyl-Gly-Gly + H2O very poor substrate Enterococcus faecalis R / ATCC 8043 benzyl alcohol + CO2 + Gly-Gly
-
 ?
3.5.1.58 N-benzyloxycarbonyl-L-Ala + H2O
-
 Enterococcus faecalis benzyl alcohol + CO2 + Ala
-
 ?
3.5.1.58 N-benzyloxycarbonyl-L-Ala + H2O
-
 Enterococcus faecalis R / ATCC 8043 benzyl alcohol + CO2 + Ala
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.58 35
-
-
 Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.58 6
-
 in 0.05 M phosphate buffer Enterococcus faecalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.58 6 6.5 stable below 20°C Enterococcus faecalis