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Literature summary extracted from

  • Shintani, Y.; Fukuda, H.; Okamoto, N.; Murata, K.; Kimura, A.
    Isolation and characterization of N-long chain acyl aminoacylase from Pseudomonas diminuta (1984), J. Biochem., 96, 637-643.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.55 2-mercaptoethanol
-
 Brevundimonas diminuta
3.5.1.55 DTT
-
 Brevundimonas diminuta
3.5.1.55 L-cysteine
-
 Brevundimonas diminuta
3.5.1.55 reduced glutathione
-
 Brevundimonas diminuta

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.55 Hg2+ HgCl2 Brevundimonas diminuta
3.5.1.55 iodoacetamide
-
 Brevundimonas diminuta
3.5.1.55 PCMB
-
 Brevundimonas diminuta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.55 1.4
-
 N-lauroyl-L-aspartate
-
 Brevundimonas diminuta
3.5.1.55 2
-
 N-stearoyl-L-glutamate
-
 Brevundimonas diminuta
3.5.1.55 2
-
 N-lauroylglycine
-
 Brevundimonas diminuta
3.5.1.55 4.9
-
 N-palmitoyl-L-glutamate
-
 Brevundimonas diminuta
3.5.1.55 5
-
 N-myristoyl-L-glutamate
-
 Brevundimonas diminuta
3.5.1.55 7.1
-
 N-lauroyl-L-glutamate
-
 Brevundimonas diminuta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.55 40000
-
 8 * 40000, enzyme I, SDS -PAGE Brevundimonas diminuta
3.5.1.55 55000
-
 4 * 55000, enzyme II, SDS-PAGE Brevundimonas diminuta
3.5.1.55 220000
-
 enzyme II, gel filtration Brevundimonas diminuta

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.55 Brevundimonas diminuta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.55 enzyme II Brevundimonas diminuta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.55 1857
-
-
 Brevundimonas diminuta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.55 additional information enzyme I is almost specific to the lipoamino acids containing L-glutamate Brevundimonas diminuta ?
-
 ?
3.5.1.55 additional information enzyme II shows broader substrate specificity than enzyme I and catalyzes the hydrolysis of lipoamino acids containing various amino acid residues Brevundimonas diminuta ?
-
 ?
3.5.1.55 N-lauroyl-L-aspartate + H2O 54% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta lauroate + L-aspartate
-
 ?
3.5.1.55 N-lauroyl-L-glutamate + H2O 85% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta lauroate + L-glutamate
-
 ?
3.5.1.55 N-lauroylglycine + H2O 17.5% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta lauroate + glycine
-
 ?
3.5.1.55 N-myristoyl-L-glutamate + H2O 91% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta myristoate + L-glutamate
-
 ?
3.5.1.55 N-myristoyl-L-glutamate + H2O 51% of the activity with N-decanoyl-L-glutamate Brevundimonas diminuta myristoate + L-glutamate
-
 ?
3.5.1.55 N-palmitoyl-L-glutamate + H2O best substrate Brevundimonas diminuta palmitate + L-glutamate
-
 ?
3.5.1.55 N-stearoyl-L-glutamate + H2O 28% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta stearate + L-glutamate
-
 ?
3.5.1.55 N-undecanoyl-L-glutamate + H2O 51% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta undecanoate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.55 octamer 8 * 40000, enzyme I, SDS -PAGE Brevundimonas diminuta
3.5.1.55 tetramer 4 * 55000, enzyme II, SDS-PAGE Brevundimonas diminuta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.55 30
-
-
 Brevundimonas diminuta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.55 40
-
 pH 7.0, 10 min, 50% loss of activity, enzyme II Brevundimonas diminuta
3.5.1.55 55
-
 pH 7, 10 min, almost complete loss of activity, enzyme II Brevundimonas diminuta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.55 6 9 substrate lauroyl-DL-valine, lauroylglycine Brevundimonas diminuta
3.5.1.55 8
-
 substrate N-lauroyl-L-glutamate, N-myristoyl-L-glutamate Brevundimonas diminuta
3.5.1.55 8
-
 N-lauroyl-L-aspartate, N-lauroyl-L-phenylalanine, N-palmitoyl-L-glutamate Brevundimonas diminuta

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.55 4 11 4°C, 24 h, stable, enzyme form II Brevundimonas diminuta