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Literature summary extracted from

  • Maitz, G.S.; Haas, E.M.; Castric, P.A.
    Purification and properties of the allophanate hydrolase from Chlamydomonas reinhardii (1982), Biochim. Biophys. Acta, 714, 486-491.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.54 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Chlamydomonas reinhardtii
3.5.1.54 acetoacetic acid Ki 0.3 mM Chlamydomonas reinhardtii
3.5.1.54 malonic acid
-
 Chlamydomonas reinhardtii
3.5.1.54 NEM allophanate protects Chlamydomonas reinhardtii
3.5.1.54 oxaloacetic acid
-
 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.54 0.55
-
 allophanate
-
 Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.54 100000
-
 gel filtration Chlamydomonas reinhardtii
3.5.1.54 110000
-
 PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.54 allophanate + H2O Chlamydomonas reinhardtii 2nd step in the degradation of urea CO2 + NH3
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.54 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.54
-
 Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.54 19.3
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.54 allophanate + H2O
-
 Chlamydomonas reinhardtii CO2 + NH3
-
 ir
3.5.1.54 allophanate + H2O 2nd step in the degradation of urea Chlamydomonas reinhardtii CO2 + NH3
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.54 9
-
-
 Chlamydomonas reinhardtii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.54 7 10 pH 7.0: about 65% of maximal activity, pH 10.0: about 30% of maximal activity Chlamydomonas reinhardtii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.54 0.3
-
 acetoacetic acid
-
 Chlamydomonas reinhardtii