EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.5 | C319A | no activity | Klebsiella aerogenes |
3.5.1.5 | C319A | 48% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value | Klebsiella aerogenes |
3.5.1.5 | C319D | 0.03% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value | Klebsiella aerogenes |
3.5.1.5 | C319S | 4.5% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value | Klebsiella aerogenes |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.5 | iodoacetamide | phosphate protects wild-type enzyme from inactivation, does not affect inactivation of C319S urease | Klebsiella aerogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.5 | 2.9 | - |
Urea | wild-type enzyme | Klebsiella aerogenes | |
3.5.1.5 | 5 | - |
Urea | mutant 319A | Klebsiella aerogenes | |
3.5.1.5 | 5.4 | - |
Urea | mutant C319D | Klebsiella aerogenes | |
3.5.1.5 | 11.3 | - |
Urea | mutant C319S | Klebsiella aerogenes |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.5 | Nickel | wild-type enzyme contains 4.6 atoms of nickel per molecule of urease, the nickel content of the mutant enzymes C319A, C319S, C319D and C319Y is lower | Klebsiella aerogenes | |
3.5.1.5 | Nickel | nickel metalloenzyme | Klebsiella aerogenes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.5 | Klebsiella aerogenes | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.5 | wild-type and mutant enzymes C319A, C319S, C319D and C319Y | Klebsiella aerogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.5 | urea + H2O | - |
Klebsiella aerogenes | CO2 + NH3 | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.5 | 5.2 | - |
mutant enzymes C319S and C319D | Klebsiella aerogenes |
3.5.1.5 | 6.7 | - |
mutant C319A | Klebsiella aerogenes |
3.5.1.5 | 7.8 | - |
wild-type enzyme | Klebsiella aerogenes |