BRENDA - Enzyme Database

Purification and properties of urease from bovine rumen

Mahadevan, S.; Sauer, F.D.; Erfle, J.D.; Biochem. J. 163, 495-501 (1977)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.5
EDTA
slight stimulation
Bos taurus
General Stability
EC Number
General Stability
Organism
3.5.1.5
dithiothreitol, 50 mM, stabilizes
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.5
Acetohydroxamate
reversible
Bos taurus
3.5.1.5
Ba2+
-
Bos taurus
3.5.1.5
Ca2+
-
Bos taurus
3.5.1.5
Cd2+
-
Bos taurus
3.5.1.5
Cu2+
-
Bos taurus
3.5.1.5
Hg2+
-
Bos taurus
3.5.1.5
Hydroxyurea
reversible
Bos taurus
3.5.1.5
Mg2+
-
Bos taurus
3.5.1.5
Mn2+
-
Bos taurus
3.5.1.5
NEM
0.1 mM, complete inhibition
Bos taurus
3.5.1.5
Ni2+
-
Bos taurus
3.5.1.5
p-chloromercuribenzene sulfonate
0.1 mM, complete inhibition
Bos taurus
3.5.1.5
Sr2+
-
Bos taurus
3.5.1.5
Zn2+
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.5
0.83
-
Urea
-
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.5
125000
-
gel filtration
Bos taurus
3.5.1.5
130000
-
sucrose density gradient centrifugation
Bos taurus
3.5.1.5
135000
-
non-denaturing PAGE
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.5
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.5
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.5
rumen
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.5
59.9
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.5
urea + H2O
-
209162
Bos taurus
CO2 + NH3
-
209162
Bos taurus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.5
8
-
-
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.5
7
8.5
pH 7.0: 52% of maximal activity, pH 8.5: 42% of maximal activity
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.5
EDTA
slight stimulation
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.1.5
dithiothreitol, 50 mM, stabilizes
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.5
Acetohydroxamate
reversible
Bos taurus
3.5.1.5
Ba2+
-
Bos taurus
3.5.1.5
Ca2+
-
Bos taurus
3.5.1.5
Cd2+
-
Bos taurus
3.5.1.5
Cu2+
-
Bos taurus
3.5.1.5
Hg2+
-
Bos taurus
3.5.1.5
Hydroxyurea
reversible
Bos taurus
3.5.1.5
Mg2+
-
Bos taurus
3.5.1.5
Mn2+
-
Bos taurus
3.5.1.5
NEM
0.1 mM, complete inhibition
Bos taurus
3.5.1.5
Ni2+
-
Bos taurus
3.5.1.5
p-chloromercuribenzene sulfonate
0.1 mM, complete inhibition
Bos taurus
3.5.1.5
Sr2+
-
Bos taurus
3.5.1.5
Zn2+
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.5
0.83
-
Urea
-
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.5
125000
-
gel filtration
Bos taurus
3.5.1.5
130000
-
sucrose density gradient centrifugation
Bos taurus
3.5.1.5
135000
-
non-denaturing PAGE
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.5
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.5
rumen
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.5
59.9
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.5
urea + H2O
-
209162
Bos taurus
CO2 + NH3
-
209162
Bos taurus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.5
8
-
-
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.5
7
8.5
pH 7.0: 52% of maximal activity, pH 8.5: 42% of maximal activity
Bos taurus