BRENDA - Enzyme Database

Purification and properties of urease from Brevibacterium ammoniagenes

Nakano, H.; Takenishi, S.; Watanabe, Y.; Agric. Biol. Chem. 48, 1495-1502 (1984)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.5
Co2+
1 mM, 39% loss of activity
Corynebacterium ammoniagenes
3.5.1.5
Cu2+
1 mM, complete inactivation
Corynebacterium ammoniagenes
3.5.1.5
Fe2+
1 mM, 42% loss of activity
Corynebacterium ammoniagenes
3.5.1.5
Hg2+
1 mM, complete inactivation
Corynebacterium ammoniagenes
3.5.1.5
Hydroxyurea
-
Corynebacterium ammoniagenes
3.5.1.5
Ni2+
1 mM, 26% loss of activity
Corynebacterium ammoniagenes
3.5.1.5
PCMB
0.1 mM, complete loss of activity
Corynebacterium ammoniagenes
3.5.1.5
Zn2+
1 mM, 36% loss of activity
Corynebacterium ammoniagenes
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.5
18
72
Urea
value depends on pH and type of buffer
Corynebacterium ammoniagenes
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.5
Nickel
contains 0.8 gatom of nickel per 67000 g of protein; contains 1 atom of nickel per subunit; nickel metalloenzyme
Corynebacterium ammoniagenes
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.5
67000
-
3 * 67000, SDS-PAGE; x * 67000, SDS-PAGE
Corynebacterium ammoniagenes
3.5.1.5
215000
-
gel filtration
Corynebacterium ammoniagenes
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.5
Corynebacterium ammoniagenes
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.5
-
Corynebacterium ammoniagenes
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.5
3500
-
-
Corynebacterium ammoniagenes
Storage Stability
EC Number
Storage Stability
Organism
3.5.1.5
20°C, 50 mM phosphate buffer, pH 7.5, 50% glycerol, 1 mM EDTA, 5 mM 2-mercaptoethanol, about 30% loss of activity after 2 months
Corynebacterium ammoniagenes
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.5
urea + H2O
-
209155
Corynebacterium ammoniagenes
CO2 + NH3
-
209155
Corynebacterium ammoniagenes
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.5
?
x * 67000, SDS-PAGE
Corynebacterium ammoniagenes
3.5.1.5
trimer
3 * 67000, SDS-PAGE
Corynebacterium ammoniagenes
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.5
60
65
-
Corynebacterium ammoniagenes
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.5
30
70
30°C: about 40% of maximal activity, 70°C: about 10% of maximal activity
Corynebacterium ammoniagenes
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.5
50
-
pH 7.5, 10 min, stable below
Corynebacterium ammoniagenes
3.5.1.5
60
-
pH 7.5, 10 min, 50% loss of activity
Corynebacterium ammoniagenes
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.5
7
-
-
Corynebacterium ammoniagenes
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.5
7
10
30°C, 5 h, stable
Corynebacterium ammoniagenes
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.5
Co2+
1 mM, 39% loss of activity
Corynebacterium ammoniagenes
3.5.1.5
Cu2+
1 mM, complete inactivation
Corynebacterium ammoniagenes
3.5.1.5
Fe2+
1 mM, 42% loss of activity
Corynebacterium ammoniagenes
3.5.1.5
Hg2+
1 mM, complete inactivation
Corynebacterium ammoniagenes
3.5.1.5
Hydroxyurea
-
Corynebacterium ammoniagenes
3.5.1.5
Ni2+
1 mM, 26% loss of activity
Corynebacterium ammoniagenes
3.5.1.5
PCMB
0.1 mM, complete loss of activity
Corynebacterium ammoniagenes
3.5.1.5
Zn2+
1 mM, 36% loss of activity
Corynebacterium ammoniagenes
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.5
18
72
Urea
value depends on pH and type of buffer
Corynebacterium ammoniagenes
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.5
Nickel
contains 0.8 gatom of nickel per 67000 g of protein; contains 1 atom of nickel per subunit; nickel metalloenzyme
Corynebacterium ammoniagenes
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.5
67000
-
3 * 67000, SDS-PAGE; x * 67000, SDS-PAGE
Corynebacterium ammoniagenes
3.5.1.5
215000
-
gel filtration
Corynebacterium ammoniagenes
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.5
-
Corynebacterium ammoniagenes
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.5
3500
-
-
Corynebacterium ammoniagenes
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.1.5
20°C, 50 mM phosphate buffer, pH 7.5, 50% glycerol, 1 mM EDTA, 5 mM 2-mercaptoethanol, about 30% loss of activity after 2 months
Corynebacterium ammoniagenes
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.5
urea + H2O
-
209155
Corynebacterium ammoniagenes
CO2 + NH3
-
209155
Corynebacterium ammoniagenes
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.5
?
x * 67000, SDS-PAGE
Corynebacterium ammoniagenes
3.5.1.5
trimer
3 * 67000, SDS-PAGE
Corynebacterium ammoniagenes
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.5
60
65
-
Corynebacterium ammoniagenes
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.5
30
70
30°C: about 40% of maximal activity, 70°C: about 10% of maximal activity
Corynebacterium ammoniagenes
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.5
50
-
pH 7.5, 10 min, stable below
Corynebacterium ammoniagenes
3.5.1.5
60
-
pH 7.5, 10 min, 50% loss of activity
Corynebacterium ammoniagenes
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.5
7
-
-
Corynebacterium ammoniagenes
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.5
7
10
30°C, 5 h, stable
Corynebacterium ammoniagenes