BRENDA - Enzyme Database

Urease from leaves of Glycine max and Zea mays

Davis, H.M.; Shih, L.M.; Phytochemistry 23, 2741-2745 (1984)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.5
Acetohydroxamate
10 mM, 88% inhibition
Glycine max
3.5.1.5
Acetohydroxamate
10 mM, 92% inhibition
Zea mays
3.5.1.5
hydroxylamine
50 mM 23% inhibition
Glycine max
3.5.1.5
hydroxylamine
50 mM, 31% inhibition
Zea mays
3.5.1.5
Hydroxyurea
10 mM, 71% inhibition
Glycine max
3.5.1.5
Hydroxyurea
10 mM, 67% inhibition
Zea mays
3.5.1.5
L-aspartyl-4-hydroxamate
50 m, 19% inhibition
Glycine max
3.5.1.5
L-aspartyl-4-hydroxamate
50 mM, 43% inhibition
Zea mays
3.5.1.5
L-glutamine
50 mM, 14% inhibition
Zea mays
3.5.1.5
L-glutamyl-5-hydroxamate
50 mM, 34% inhibition
Zea mays
3.5.1.5
L-Gly hydroxamate
1 mM, 97% inhibition
Glycine max
3.5.1.5
L-Gly hydroxamate
1 mM, 94% inhibition
Zea mays
3.5.1.5
Selenourea
50 mM, 80% inhibition
Glycine max
3.5.1.5
Semicarbazide
50 mM, 48% inhibition
Glycine max
3.5.1.5
Semicarbazide
50 mM, 31% inhibition
Zea mays
3.5.1.5
Thiourea
50 mM, 26% inhibition
Glycine max
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.5
0.45
-
Urea
pH 5.5
Zea mays
3.5.1.5
0.5
-
Urea
pH 5.5
Glycine max
3.5.1.5
0.5
-
Urea
pH 8.8
Zea mays
3.5.1.5
0.9
-
Urea
pH 8.8
Glycine max
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.5
Glycine max
-
-
-
3.5.1.5
Zea mays
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.5
leaf
-
Glycine max
-
3.5.1.5
leaf
-
Zea mays
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.5
urea + H2O
-
209153
Zea mays
CO2 + NH3
-
209153
Zea mays
-
3.5.1.5
urea + H2O
-
209153
Glycine max
CO2 + NH3
-
209153
Glycine max
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.5
5.5
-
and a second higher optimum at pH 9.0
Glycine max
3.5.1.5
5.5
-
and 2 other optima at pH 7.5 and 8.8
Zea mays
3.5.1.5
7.5
-
and 2 other optima at pH 5.5 and pH 8.8
Zea mays
3.5.1.5
8.8
-
and 2 lower optima at pH 5.5 and pH 7.5
Zea mays
3.5.1.5
9
-
and a second lower optimum at pH 5.5
Glycine max
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.5
Acetohydroxamate
10 mM, 88% inhibition
Glycine max
3.5.1.5
Acetohydroxamate
10 mM, 92% inhibition
Zea mays
3.5.1.5
hydroxylamine
50 mM 23% inhibition
Glycine max
3.5.1.5
hydroxylamine
50 mM, 31% inhibition
Zea mays
3.5.1.5
Hydroxyurea
10 mM, 71% inhibition
Glycine max
3.5.1.5
Hydroxyurea
10 mM, 67% inhibition
Zea mays
3.5.1.5
L-aspartyl-4-hydroxamate
50 m, 19% inhibition
Glycine max
3.5.1.5
L-aspartyl-4-hydroxamate
50 mM, 43% inhibition
Zea mays
3.5.1.5
L-glutamine
50 mM, 14% inhibition
Zea mays
3.5.1.5
L-glutamyl-5-hydroxamate
50 mM, 34% inhibition
Zea mays
3.5.1.5
L-Gly hydroxamate
1 mM, 97% inhibition
Glycine max
3.5.1.5
L-Gly hydroxamate
1 mM, 94% inhibition
Zea mays
3.5.1.5
Selenourea
50 mM, 80% inhibition
Glycine max
3.5.1.5
Semicarbazide
50 mM, 48% inhibition
Glycine max
3.5.1.5
Semicarbazide
50 mM, 31% inhibition
Zea mays
3.5.1.5
Thiourea
50 mM, 26% inhibition
Glycine max
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.5
0.45
-
Urea
pH 5.5
Zea mays
3.5.1.5
0.5
-
Urea
pH 5.5
Glycine max
3.5.1.5
0.5
-
Urea
pH 8.8
Zea mays
3.5.1.5
0.9
-
Urea
pH 8.8
Glycine max
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.5
leaf
-
Glycine max
-
3.5.1.5
leaf
-
Zea mays
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.5
urea + H2O
-
209153
Zea mays
CO2 + NH3
-
209153
Zea mays
-
3.5.1.5
urea + H2O
-
209153
Glycine max
CO2 + NH3
-
209153
Glycine max
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.5
5.5
-
and a second higher optimum at pH 9.0
Glycine max
3.5.1.5
5.5
-
and 2 other optima at pH 7.5 and 8.8
Zea mays
3.5.1.5
7.5
-
and 2 other optima at pH 5.5 and pH 8.8
Zea mays
3.5.1.5
8.8
-
and 2 lower optima at pH 5.5 and pH 7.5
Zea mays
3.5.1.5
9
-
and a second lower optimum at pH 5.5
Glycine max