Literature summary extracted from

Wyborn, N.R.; Mills, J.; Williams, S.G.; Jones, C.W.
Molecular characterization of formamidase from Methylophilus methylotrophus (1996), Eur. J. Biochem., 240, 314-322.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.5.1.49	Thiourea	competitive inhibition, Ki: 4.6 mM	Methylophilus methylotrophus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.5.1.49	44481	-	3 * 44481, electrospray mass spectrometry	Methylophilus methylotrophus
3.5.1.49	133000	137000	gel filtration, non-denaturing PAGE	Methylophilus methylotrophus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.1.49	Methylophilus methylotrophus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.1.49	to homogeneity	Methylophilus methylotrophus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.5.1.49	cell culture	-	Methylophilus methylotrophus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.1.49	formamide + H2O	-	Methylophilus methylotrophus	formate + NH3	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.5.1.49	trimer	3 * 44481, electrospray mass spectrometry	Methylophilus methylotrophus

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.5.1.49	4.6	-	Thiourea	-	Methylophilus methylotrophus

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Release 2023.1 (January 2023)