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Literature summary extracted from

  • Skouloubris, S.; Labigne, A.; de Reuse H.
    Identification and characterization of an aliphatic amidase in Helicobacter pylori (1997), Mol. Microbiol., 25, 989-998.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.4 expression in Escherichia coli, strain N6, N6-ureB::TnKm-4 and N6-836 Helicobacter pylori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.4 cytoplasm
-
 Helicobacter pylori 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 49000
-
 SDS-PAGE Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Helicobacter pylori
-
-
-
3.5.1.49 Helicobacter pylori
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.49 cell culture
-
 Helicobacter pylori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 0.2
-
 strain N6-836, formamide, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 0.2
-
 strain N6-ureB::TnKm-4, formamide, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 0.23
-
 strain N6, isobutyramide, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 0.25
-
 strain N6, formamide, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 0.35
-
 strain N6-ureB::TnKm-4, isobutyramide, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 6.5
-
 strain N6, acetamide, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 11
-
 strain N6, acrylamide, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 12
-
 strain N6, propionamide, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 17
-
 strain N6-ureB::TnKm-4, acetamide, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 28
-
 strain N6-ureB::TnKm-4, acrylamide, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 28
-
 strain N6-ureB::TnKm-4, propionamide, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 50
-
 strain N6, urea, pH 7.4, room temperature, 30 min Helicobacter pylori
3.5.1.4 65
-
 strain N6-836, urea, pH 7.4, room temperature, 30 min Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 acetamide + H2O
-
 Helicobacter pylori acetic acid + NH3
-
 ?
3.5.1.4 acrylamide + H2O
-
 Helicobacter pylori prop-2-enoic acid + NH3
-
 ?
3.5.1.4 propionamide + H2O
-
 Helicobacter pylori propionic acid + NH3
-
 ?
3.5.1.49 formamide + H2O from AmiE gene, very low activity Helicobacter pylori formate + NH3
-
 ?